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Publication Icon   Literature - Publication Review
HTRF solutions for cytokines in autoimmune inflammation research
Discover publications using cytokine read-outs to screen drug targets in autoimmune diseases.
Technical Note Icon   Technical Note
Guidelines from PBMC isolation to cytokine assay optimization
This tech note will provide you with a complete step by step methodology to purify PBMCs and use them in cytokine quantification assays with HTRF
Application Note Icon   Application Note
Inhibition of FGFR signaling pathways studied in cancer cell lines using HTRF
In this application note, we show that a pan-FGFR inhibitor, AZD4547, used in different clinical phases depending on the cancer type, is able to inhibit or even suppress the abnormal phosphorylation of FGFRs and their key effectors in 3 different types of cancer cell lines: KG-1 (blood cancer), SNU-16 (gastric cancer), and KMS-11 (multiple myeloma).
Guide Icon   Guide
The ultimate guide to PPI assays with HTRF
This User Guide reviews the general principles and steps in developing a protein-protein interaction assay using HTRF PPi reagents from Revvity.
Publication Icon   Literature - Publication Review
GPCR signaling in cardiovascular research addressed with HTRF second messenger assays
This note compiles published examples of cardiovascular related studies in which cAMP and IP-One HTRF second messengers assays were successfully used to monitor Gi/s and Gq activity and allowed for a better understanding or addressing of CVDs.
Application Note Icon   Application Note
HTRF assays handle low- to high affinity protein-protein interactions
This application note demonstrated that HTRF PPI reagents are successfully used combining high detection performance, accurate modulators screening, and pharmacological characterization.
Other Icon   Other
Practice License of Medical Institution
Lab license certificate to perform medical tests.
Application Note Icon   Application Note
Kinetic Binding of Kinase inhibitors and determination of Kon, Koff rates
This application note presents the use of fluorescent kinase inhibitors to determine the kinetic binding properties of inhibitors of interest and demonstrates the pharmacological relevance of the kinase binding platform for both equilibrium-and kinetic profiling.
Application Note Icon   Application Note
Characterizing pharmacological compounds with complementary Upstream and Downstream GPCR readouts
This application note presents and compares the pharmacological characterization of a panel of reference compounds (full agonists, partial agonists, antagonists) in δ-opioid and D2 receptor models with two different Gi-coupled receptor activity monitoring assays: the HTRF® GTP Gi binding assay and the HTRF cAMP Gi assay.
Technical Note Icon   Technical Note
Guidelines for optimizing protein-protein interaction assays using HTRF PPI reagents
This technical note reviews the general principles and steps in developing a protein-protein interaction assay using HTRF PPi reagents from Revvity assays.
Publication Icon   Literature - Publication Review
Literature review on solutions for investigating Huntington’s disease
This literature review shows how researchers have taken advantage of immunoassays to move their research forward. It features experiments and data from published studies that investigated new leads for Huntington’s disease drug discovery.
Publication Icon   Literature - Publication Review
Key signaling pathways in liquid cancer: therapeutic targets, screening tools, and future developments
This review highlights some of these signaling pathways and demonstrates how assays based on HTRF and AlphaLISA technologies can be used in the screening and development of new therapies for leukemia and lymphoma treatment.
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