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Technical Note

Guidelines from PBMC isolation to cytokine assay optimization

Perform and optimize cytokine assays on PBMC

This tech note will provide you with a complete step by step methodology to purify PBMCs and use them in cytokine quantification assays with HTRF. Take a good look at the protocols and examples it features!

Included

  • PBMC isolation protocol
  • HTRF basics and cytokine assays
  • Optimization and examples

For research use only. Not for use in diagnostic procedures.

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Guidelines from PBMC isolation to cytokine assay optimization