ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption & disposal requirements under European REACH regulations (EC 1907/2006).
ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
ATPlite™ is an Adenosine TriPhosphate (ATP) monitoring system based on firefly (Photinus pyralis) luciferase. This luminescence assay is the alternative to colorimetric, fluorometric and radioisotopic assays for the quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. ATP monitoring can be used to assess the cytocidal, cytostatic and proliferative effects of a wide range of drugs, biological response modifiers and biological compounds.
Advantages:
Assay Points |
300
|
---|---|
Assay Protocol |
2-step
|
Automation Compatible |
Yes
|
Brand |
ATPlite
|
Cellular Process |
Autophagy
Cell Death
Cell Growth
Cytotoxicity
Necrosis
|
Detection Method |
Luminescence
|
Experimental Type |
In vitro
|
Format |
Microplates
|
One Unit Contains |
300.0 assay points
|
Shipping Conditions |
Shipped Ambient
|
Unit Size |
300 (96-well) / 1200 (384-well)
|
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In this application note three ATP monitoring luminescence assays were assessed and compared head-to-head: (i) ATPlite™ from...
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