Reporter gene assays
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells.
Reporter gene assays enable high sensitivity measurement of gene expression and cell signaling through the addition of bioluminescent genes into target cells.
Our reporter gene assay reagents produce a long-lived glow for plate batch processing and simplified, automated, cost-effective screens. Our luciferase assay reagent options have a half-life of several hours, allowing for streamlined detection procedures.
Use our reporter assays to characterize the strength of promoters and enhancers, define the role of transcription factors, or assess transfection efficiency during drug development. Benefits include:
- Convenient one-step processing: No washes required – simply mix, incubate, and read
- Strong signal for remarkably accurate and thorough firefly or renilla luciferase reading
- Enhanced stability: All our luciferase reporter assay options are designed for extended storage at 2-8°C
- No DTT: Unlike most luciferase detection reagents, our reporter gene assay options do not contain DTT, eliminating toxic hazards and the need for hood work
- Amenable to high throughput and ultra-high throughput environments, using 384- or 1536-well microplate formats
- Excellent Z' Values: Each luciferase assay system offers robust, sensitive performance, with high signal-to-background ratios
Our luciferase reporter assay options include:
- britelite™ plus: ideal for assays requiring the utmost sensitivity
- neolite™: this reporter assay offers the ultimate compromise between strong signal intensity and extended signal half-life
- steadylite™ plus: the reagent of choice for HTS where you require the same steady signal first plate into last plate out
- sensilite™: a reporter assay designed to provide maximum signal intensity
- twinlite™: ideal reporter assay for normalization for high quality data and to screen two events in parallel
Whether your reporter assay requires the sensitivity provided by high signal intensity, or the flexibility of an extended signal half-life, Revvity offers a reporter assay to suit your needs.
Cytotoxicity and cell proliferation assays
Cytotoxicity and cell proliferation assays are commonly used in the drug discovery process to assess a compound’s ability to cause or block a biologic activity without having toxic effects on cells. Adenosine TriPhosphate (ATP) – monitoring assays allow for the quantitative evaluation of proliferation and cytotoxicity.
Cytotoxicity and cell proliferation assays are commonly used in the drug discovery process to assess a compound’s ability to cause or block a biologic activity without having toxic effects on cells. Adenosine TriPhosphate (ATP) – monitoring assays allow for the quantitative evaluation of proliferation and cytotoxicity.
Our ATP luminescence assays provide a more sensitive alternative to colorimetric, fluorometric, and radioisotopic based assays for monitoring cell viability and proliferation. These innovative, patented technologies are based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
Choose our ATPlite™ 1step assay for a single addition assay and our ATPlite assay for extended signal stability.
Kinase activity
For fast, reliable detection of any kinase activity in a one-step, homogeneous assay format.
For fast, reliable detection of any kinase activity in a one-step, homogeneous assay format.
