Virus blockade assay
Adapted from Newton et al. / Journal of Biomolecular Screening 18(3) (2012) 237–246.
A wide range of biomolecular interactions can be assessed
The advances in identifying short primary sequences mostly involved in the PPI interfaces, also called hot-spots, have greatly facilitated the setup of biochemical assays to identify PPI modulators. However in some projects the binding epitopes involved in PPI interfaces are not fully understood, which requires the use of full size proteins to reproduce the interaction and screen for inhibitors or stabilizers.
HTRF is a popular technology for PPIs, whatever the molecular weight of your protein partners, as illustrated below:
Large size complex
B-Catenin/TCF4
This study was performed to show the ability of HTRF PPI reagents to address large biomolecular interactions. Recombinant human GST-β-Catenin (115 KDa) and 6HIS-TCF4 (55 KDa) interaction was detected using Anti GST-Eu Cryptate (150 KDa) and Anti 6HIS-d2 (150 KDa) antibodies.
HTRF is suitable for low to high affinity interactions
Protein-Protein interaction networks are extended and complex. They are also conveyed by a vast number of non-covalent binding modes. This diversity results in a wide range of affinities from very weak and transient interactions to very strong (High µM to picomolar).
The reliability of HTRF, coupled with a wide diversity of PPI tools, helps to enable the efficient measurement of complex interactions displaying low to high Kd.
HTRF PPI assays taken from published papers, displaying a large panel of affinities.
Let’s see how to get started with your own PPI!
-
Choose your target interaction
Receptor/ligands, RNA, DNA, lipids, enzymes, etc. Any interacting proteins are within your reach: It’s all about what you want!
-
Select assay format & starting material
Antibodies, native partner, biotinylated partner, tagged protein, Fc fused proteins matching your goals.
-
Choose your detection reagents
46 options ensure that you find a match for your interaction: labeling kits, affinity reagents, detection and anti species reagents.
-
Assay optimization and data processing
A single optimization step to adjust reagents to each other.
And off to the reader you go!
Receptor-Ligand binding assay
Adapted from C J. Rossant / Journal of Biomolecular Screening 2015, Vol. 20(4) 508–518
A wide range of biomolecular interactions can be assessed
The advances in identifying short primary sequences mostly involved in the PPI interfaces, also called hot-spots, have greatly facilitated the setup of biochemical assays to identify PPI modulators. However in some projects the binding epitopes involved in PPI interfaces are not fully understood, which requires the use of full size proteins to reproduce the interaction and screen for inhibitors or stabilizers.
HTRF is a popular technology for PPIs, whatever the molecular weight of your protein partners, as illustrated below:
Large size complex
B-Catenin/TCF4
This study was performed to show the ability of HTRF PPI reagents to address large biomolecular interactions. Recombinant human GST-β-Catenin (115 KDa) and 6HIS-TCF4 (55 KDa) interaction was detected using Anti GST-Eu Cryptate (150 KDa) and Anti 6HIS-d2 (150 KDa) antibodies.
HTRF is suitable for low to high affinity interactions
Protein-Protein interaction networks are extended and complex. They are also conveyed by a vast number of non-covalent binding modes. This diversity results in a wide range of affinities from very weak and transient interactions to very strong (High µM to picomolar).
The reliability of HTRF, coupled with a wide diversity of PPI tools, helps to enable the efficient measurement of complex interactions displaying low to high Kd.
HTRF PPI assays taken from published papers, displaying a large panel of affinities.
Let’s see how to get started with your own PPI!
-
Choose your target interaction
Receptor/ligands, RNA, DNA, lipids, enzymes, etc. Any interacting proteins are within your reach: It’s all about what you want!
-
Select assay format & starting material
Antibodies, native partner, biotinylated partner, tagged protein, Fc fused proteins matching your goals.
-
Choose your detection reagents
46 options ensure that you find a match for your interaction: labeling kits, affinity reagents, detection and anti species reagents.
-
Assay optimization and data processing
A single optimization step to adjust reagents to each other.
And off to the reader you go!
mRNA/Protein binding assay
Adapted from M.D.Disney et al. / ACS Chem Biol. 2012 October 19; 7(10): 1711–1718
A wide range of biomolecular interactions can be assessed
The advances in identifying short primary sequences mostly involved in the PPI interfaces, also called hot-spots, have greatly facilitated the setup of biochemical assays to identify PPI modulators. However in some projects the binding epitopes involved in PPI interfaces are not fully understood, which requires the use of full size proteins to reproduce the interaction and screen for inhibitors or stabilizers.
HTRF is a popular technology for PPIs, whatever the molecular weight of your protein partners, as illustrated below:
Large size complex
B-Catenin/TCF4
This study was performed to show the ability of HTRF PPI reagents to address large biomolecular interactions. Recombinant human GST-β-Catenin (115 KDa) and 6HIS-TCF4 (55 KDa) interaction was detected using Anti GST-Eu Cryptate (150 KDa) and Anti 6HIS-d2 (150 KDa) antibodies.
HTRF is suitable for low to high affinity interactions
Protein-Protein interaction networks are extended and complex. They are also conveyed by a vast number of non-covalent binding modes. This diversity results in a wide range of affinities from very weak and transient interactions to very strong (High µM to picomolar).
The reliability of HTRF, coupled with a wide diversity of PPI tools, helps to enable the efficient measurement of complex interactions displaying low to high Kd.
HTRF PPI assays taken from published papers, displaying a large panel of affinities.
Let’s see how to get started with your own PPI!
-
Choose your target interaction
Receptor/ligands, RNA, DNA, lipids, enzymes, etc. Any interacting proteins are within your reach: It’s all about what you want!
-
Select assay format & starting material
Antibodies, native partner, biotinylated partner, tagged protein, Fc fused proteins matching your goals.
-
Choose your detection reagents
46 options ensure that you find a match for your interaction: labeling kits, affinity reagents, detection and anti species reagents.
-
Assay optimization and data processing
A single optimization step to adjust reagents to each other.
And off to the reader you go!
Antibody epitope mapping
Adapted from C J. Rossant / Journal of Biomolecular Screening 2015, Vol. 20(4) 508–518
A wide range of biomolecular interactions can be assessed
The advances in identifying short primary sequences mostly involved in the PPI interfaces, also called hot-spots, have greatly facilitated the setup of biochemical assays to identify PPI modulators. However in some projects the binding epitopes involved in PPI interfaces are not fully understood, which requires the use of full size proteins to reproduce the interaction and screen for inhibitors or stabilizers.
HTRF is a popular technology for PPIs, whatever the molecular weight of your protein partners, as illustrated below:
Large size complex
B-Catenin/TCF4
This study was performed to show the ability of HTRF PPI reagents to address large biomolecular interactions. Recombinant human GST-β-Catenin (115 KDa) and 6HIS-TCF4 (55 KDa) interaction was detected using Anti GST-Eu Cryptate (150 KDa) and Anti 6HIS-d2 (150 KDa) antibodies.
HTRF is suitable for low to high affinity interactions
Protein-Protein interaction networks are extended and complex. They are also conveyed by a vast number of non-covalent binding modes. This diversity results in a wide range of affinities from very weak and transient interactions to very strong (High µM to picomolar).
The reliability of HTRF, coupled with a wide diversity of PPI tools, helps to enable the efficient measurement of complex interactions displaying low to high Kd.
HTRF PPI assays taken from published papers, displaying a large panel of affinities.
Let’s see how to get started with your own PPI!
-
Choose your target interaction
Receptor/ligands, RNA, DNA, lipids, enzymes, etc. Any interacting proteins are within your reach: It’s all about what you want!
-
Select assay format & starting material
Antibodies, native partner, biotinylated partner, tagged protein, Fc fused proteins matching your goals.
-
Choose your detection reagents
46 options ensure that you find a match for your interaction: labeling kits, affinity reagents, detection and anti species reagents.
-
Assay optimization and data processing
A single optimization step to adjust reagents to each other.
And off to the reader you go!
Protease assay
Ingo H. Engels et al. / Analytical Biochemistry 390 (2009) 85–87
A wide range of biomolecular interactions can be assessed
The advances in identifying short primary sequences mostly involved in the PPI interfaces, also called hot-spots, have greatly facilitated the setup of biochemical assays to identify PPI modulators. However in some projects the binding epitopes involved in PPI interfaces are not fully understood, which requires the use of full size proteins to reproduce the interaction and screen for inhibitors or stabilizers.
HTRF is a popular technology for PPIs, whatever the molecular weight of your protein partners, as illustrated below:
Large size complex
B-Catenin/TCF4
This study was performed to show the ability of HTRF PPI reagents to address large biomolecular interactions. Recombinant human GST-β-Catenin (115 KDa) and 6HIS-TCF4 (55 KDa) interaction was detected using Anti GST-Eu Cryptate (150 KDa) and Anti 6HIS-d2 (150 KDa) antibodies.
HTRF is suitable for low to high affinity interactions
Protein-Protein interaction networks are extended and complex. They are also conveyed by a vast number of non-covalent binding modes. This diversity results in a wide range of affinities from very weak and transient interactions to very strong (High µM to picomolar).
The reliability of HTRF, coupled with a wide diversity of PPI tools, helps to enable the efficient measurement of complex interactions displaying low to high Kd.
HTRF PPI assays taken from published papers, displaying a large panel of affinities.
Let’s see how to get started with your own PPI!
-
Choose your target interaction
Receptor/ligands, RNA, DNA, lipids, enzymes, etc. Any interacting proteins are within your reach: It’s all about what you want!
-
Select assay format & starting material
Antibodies, native partner, biotinylated partner, tagged protein, Fc fused proteins matching your goals.
-
Choose your detection reagents
46 options ensure that you find a match for your interaction: labeling kits, affinity reagents, detection and anti species reagents.
-
Assay optimization and data processing
A single optimization step to adjust reagents to each other.
And off to the reader you go!
