Alpha
Alpha is a bead-based, no-wash, luminescent technology that offers a faster, microplate-based alternative to ELISA. When Alpha Donor and Acceptor beads are brought together through binding to target, a cascade of chemical reactions are set in motion, creating an amplified signal. These versatile beads are conjugated with various biomolecules that enable detection of unique biological events. Alpha technology is available in:
- AlphaLISA™ – Products are available as either ready-to-use kits to quantify biomarkers and cytokines, or standalone toolbox products to develop your own assay.
- AlphaLISA™ SureFire® Ultra™ – Kits developed to measure total and phosphorylated protein levels in cell lysates, these products are an excellent choice for studying cell signaling pathways or endogenous receptors
Alpha offers benefits such as:
- Faster time to results
- Options for multiplexing
- Ability to quantitate large protein complexes, nucleosomes, and viral particles
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Compatible with a wide range of sample types, including simple buffers, cell supernatants, cell lysates, complex tissue, serum, and plasma
DELFIA TRF
DELFIA® TRF is a proven, robust wash-based immunodetection platform with over 20 years of history. DELFIA has a similar assay principle and workflow to that of a traditional ELISA, but with the added benefits of a stable, time-resolved fluorescent signal improved assay dynamic range. DELFIA also offers the ability for multiplexing. DELFIA TRF is compatible with the widest range of sample types, ranging from simple buffers, cell supernatants, and cell lysates to complex biological samples, including tissue, serum, plasma, and blood.
ELISA
Focus on a single target with BioLegend’s ELISA kits and sets. Find all the reagents for your ELISA workflow with our economical kits, antibodies, and ancillary reagents. BioLegend offers four types of ELISA kits, including easy-to-use LEGEND MAX™ kits with pre-coated plates and RAPID MAX™ kits that cut your assay time down to 90 minutes (watch the protocol video to learn how time is saved). In addition, BioLegend provides reliable antibody pairs, ancillary reagents, and standards for those who want to develop their own ELISA assays.
HTRF
HTRF® (Homogeneous Time Resolved Technology) is a FRET-based, no-wash technology.
Rapid, homogeneous, easy to use and to automate, allowing extreme miniaturization and easy assay development – over time, HTRF has become the gold reference for time-resolved FRET.
HTRF technology can be used in competitive and noncompetitive formats, performed as cellular or biochemical assays and thus can been applied to a variety of applications including GPCRs, kinases, epigenetics, and quantification of a range of biomarkers including cytokines.
LANCE Ultra TR-FRET
LANCE® Ultra TR-FRET is a no-wash proximity assay technology that combines the benefits of time resolution (TR) with fluorescence resonance energy transfer (FRET) principles by using energy donor and energy acceptor fluorophore labels. It’s everything you love about TRF detection with fastest results and no wash steps. LANCE Ultra TR-FRET is compatible with buffer, cellular supernatant and cell lysate samples.
LEGENDplex
LEGENDplex™ bead-based immunoassays work on the same principle as a sandwich ELISA, with the advantage that they can simultaneously quantify up to 14 targets in a single sample using only a flow cytometer - no other instruments are needed. Data can be analyzed with our free LEGENDplex Data Analysis Software Suite. Choose from dozens of existing panels or customize your own.
Explore Alpha, TRF, and TR-FRET by therapeutic area
Virology reagents
Neuroscience research reagents
Immuno-oncology reagents
Explore Alpha, TRF, and TR-FRET by target area
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