Genetic testing and biobanking
Genomic DNA from diverse sample types for genetic workflows and biobanking
Genomic DNA from diverse sample types for genetic workflows and biobanking
The high, archival integrity of nucleic acids obtained with chemagic technology is ideal for biobanks and testing laboratories. The chemagic separation technology automates the efficient extraction of high molecular weight (HMW) DNA, enabling successful application in diverse downstream assays for genetic testing such as HLA typing1 for donor screening, SNP arrays for pharmacogenetics2, MLPA for copy number detection3, NGS from dried blood spots for newborn research4 and long read sequencing in genetic research5.
“More than 10 years ago, we chose chemagen instruments and reagents for isolating DNA from blood or buccal swabs. After performing HLA genotyping on over 10 million samples, we remain extremely satisfied with this decision and the consistent performance.”
Dr. Vinzenz Lange, CTO, DKMS Life Science Lab, Germany
- Schöfl G, Lang K, Quenzel P, et al. (2017). 2.7 million samples genotyped for HLA by next generation sequencing: lessons learned. BMC Genomics 18:1, 18(1), 1–16. https:// doi.org/10.1186/S12864-017-3575-Z3.
- Macha S, Koenen R, Sennewald R, et al. (2014). Effect of Gemfibrozil, Rifampicin, or Probenecid on the Pharmacokinetics of the SGLT2 Inhibitor Empagliflozin in Healthy Volunteers. Clinical Therapeutics, 36(2), 280-290.e1. https://doi.org/10.1016/j.clinthera.2014.01.003
- Evans DG, Lalloo F, Ryan NA, Bowers N, Green K, Woodward ER et al. (2022). Advances in genetic technologies result in improved diagnosis of mismatch repair deficiency in colorectal and endometrial cancers. Journal of Medical Genetics, 59(4), 328–334. https://doi.org/10.1136/JMEDGENET-2020-107542
- Balciuniene, Jorune et al. (2023). At-Risk Genomic Findings for Pediatric-Onset Disorders From Genome Sequencing vs Medically Actionable Gene Panel in Proactive Screening of Newborns
- Beyter D, Ingimundardottir H, Oddsson A, et al. (2021). Long-read sequencing of 3,622 Icelanders provides insight into the role of structural variants in human diseases and other traits. Nature Genetics, 53(6), 779–786. https://doi.org/10.1038/s41588-021-00865-4and Children. JAMA Network Open, 6(7), e2326445–e2326445. https://doi.org/10.1001/JAMANETWORKOPEN.2023.26445
Precision oncology
cfDNA from liquid biopsies
cfDNA from liquid biopsies
The unique large volume processing capability of chemagic instruments and high specificity of M-PVA Magnetic Beads combine to boost extraction yields of cfDNA from liquid biopsies, increasing chances of detection downstream. Easily scale from low to high throughputs and manual to automated kit options. chemagic cfDNA extraction has been used to support the clinical research of colorectal6, lung7, prostate and anal cancer8 with downstream methods including digital PCR and pan-cancer NGS panels.
“We use cfDNA extracted with chemagic™ technology in digital droplet PCR (ddPCR) assays to support research of colorectal cancer, lung cancer, and anal cancer (based on HPV cfDNA detection) with a sample to result turnaround time down to 24 h.”
Professor Niels Pallisgaard, Dept of Pathology, Zealand University Hospital, Denmark
6. Callesen LB, Hansen TF, Andersen RF, et al. (2023). ctDNA-guided adjuvant treatment after radical-intent treatment of metastatic spread from colorectal cancer-the first interim results from the OPTIMISE study. Acta Oncologica (Stockholm, Sweden), 62(12), 1742–1748.
7. Frank MS, Andersen CSA, Ahlborn LB, Pallisgaard N, Bodtger U, Gehl J. (2022). Circulating Tumor DNA Monitoring Reveals Molecular Progression before Radiologic Progression in a Real-life Cohort of Patients with Advanced Non–small Cell Lung Cancer. Cancer Research Communications, 2(10), 1174–1187. https://doi.org/10.1158/2767- 9764.CRC-22-0258
8. Lefèvre AC, Pallisgaard N, Kronborg C, Wind KL, Krag SRP, Spindler KG. (2021). The clinical value of measuring circulating hpv dna during chemo-radiotherapy in squamous cell carcinoma of the anus. Cancers, 13(10). https://doi.org/10.3390/cancers13102451
DNA from frozen/FFPE tissue, blood, or bone marrow
To support the growing demand, nucleic acid extraction from blood, bone marrow cells, or tumor tissue that is either fresh, frozen, or formalin-fixed, paraffin-embedded (FFPE) is often performed. The chemagic kits consistently produce high-quality, NGS grade DNA and RNA from these sample types and more. Commonly cited downstream applications include RT-PCR, methylation arrays, MLPA and pan-cancer NGS panels9,10.
9. Benhamida JK, Hechtman JF, Nafa K, et al. (2020). Reliable Clinical MLH1 Promoter Hypermethylation Assessment Using a High-Throughput Genome-Wide Methylation Array Platform. The Journal of Molecular Diagnostics, 22(3), 368–375. https://doi.org/10.1016/J.JMOLDX.2019.11.005
10. Sorrells S, McKinnon KE, McBratney A, Sumey C. (2021). Longitudinal and multi-tissue molecular diagnostics track somatic BRCA2 reversion mutations that correct the open reading frame of germline alteration upon clinical relapse. Npj Genomic Medicine 2021 6:1, 6(1), 1–6. https://doi.org/10.1038/s41525-021-00181-0 25-021-00181-0
Translational research and biomarker development
RNA from cells, tissue, and blood
RNA from cells, tissue, and blood
The chemagic RNA isolation technology has been applied to various sample types including blood, tissues, and cells and provides researchers consistent high integrity RNA suitable for a variety of downstream applications including RNA sequencing11.
11. Schieck, Maximilian et al. (2020) Implementation of RNA sequencing and array CGH in the diagnostic workflow of the AIEOP-BFM ALL 2017 trial on acute lymphoblastic leukemia. Annals of hematology vol. 99(4) 809–818. https://doi.org/10.1007/s00277-020-03953-3
miRNA from plasma, serum, or exosomes
Despite the small size and low concentrations of cell free circulating microRNA (miRNA), the chemagic miRNA kits obtain high, consistent yields comparable to manual methods with only 10 minutes of hands-on time. Suitable for plasma, serum, extracellular vesicles and saliva, miRNAs obtained have been analyzed by rtqPCR and NGS.
“We have started the testing of cfRNA isolations with our chemagic 360 instrument – the RNA yields appear fine from different plasma and serum samples; thus we look forward to use the kit for high throughput applications.”
Dr. Maija Puhka, Head of HiPrep and EV Core, Institute for Molecular Medicine RNA from cells, tissue, and blood
Infectious disease research
Pathogen nucleic acids from body fluids
Pathogen nucleic acids from body fluids
Fast turn-around times, diverse sample type processing and high, consistent yields make chemagic nucleic acid kits with chemagic instruments the choice for many research labs working on infectious diseases. Automated extraction workflows from gram positive and negative bacteria, intra and extracellular viruses, yeast, funghi, and protozoa have been performed from body fluids like saliva, sputum, serum, plasma, lavage, urine, semen, amniotic fluid as well as swabs in various transport media. Challenging sample types such as stool, ticks, cultures, and wastewater have also been processed. Applications cited have been wide-ranging from blood-borne parasite screening to wastewater surveillance of infectious agents12,13,14.
“The chemagic 360 instrument has been functioning well, satisfying the requirements of our COVID-19 testing (4000 samples/day) and meeting our expectations for quality and reliability.“
Department of Microbiology, Government of Rajasthan, S.M.S. Medical School, Jaipur
12. Costa E, Rocha D, Lopes JIF, et al. (2024). Detection of Plasmodium spp. in asymptomatic blood donors by the new Brazilian NAT PLUS HIV/HBV/HCV/Malaria Bio Manguinhos kit. Transfusion, 64(3), 501–509. https://doi.org/10.1111/TRF.17726
13. LaTurner, Zachary W et al. (2021) Evaluating recovery, cost, and throughput of different concentration methods for SARS-CoV-2 wastewater-based epidemiology” Water research vol. 197: 117043. doi:10.1016/j.watres.2021.117043 (2021). Evaluating recovery, cost, and throughput of different concentration methods for SARS-CoV-2 wastewater-based epidemiology. Water Research, 197. (2021).
14. West NW, Vasquez AA, Bahmani A, et al. (2022) Sensitive detection of SARS-CoV-2 molecular markers in urban community sewersheds using automated viral RNA purification and digital droplet PCR. Sci Total Environ. 847:157547. doi:10.1016/j.scitotenv.2022.15754
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