Omni Bead Ruptor bead mill homogenizer protocols
Various tissues and sample types were homogenized and assessed for analytes using the portfolio of Omni Bead Ruptor bead mill homogenizers. Below is our list of resulting performance-verified protocol snapshots. Note that all listed protocols are only recommended starting points from our or external lab experiences, and labs will need to verify their own protocols for their own workflows.
For research use only. Not for use in diagnostic procedures.
Sample Types
Human (Homo sapien) tissue
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Blood | 0.8 mL | Protein, DNA | 0.1 mm ceramic | 2 mL tube, screw cap |
1. 30 Hz for 2.5 mins, 2 cycles Note: Flip tube holder 180 ° after each cycle |
Bead Ruptor 96 | 19-632 |
Teeth | One tooth | DNA | 2 x 10 mm stainless steel milling ball | 25 mL milling jar |
1. 20 Hz, 1 min Note: Place jar in freezer for 10 min prior to use |
Bead Ruptor 96 | 27-203; 27-004 |
Animal tissue
Mammals
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Bat skin (Artibeus phaeotis) | 25 – 100 mg | Amino acids | 2.8 mm ceramic | 7 mL tube | 1. 6.8 m/s for 30 sec 2. Add 3 mL of TCA to tube 3. 6.8 m/s, 30 secs, 3 additional cycles Note: Trichloroacetic acid (TCA) is a protein-precipitating agent. |
Bead Ruptor Elite | 19-678 |
Bull hair (Bos Taurus) | 50 mg | Steroids | 2.4 mm metal | 2 mL reinforced tube | 1. 6.95 m/s for 50 secs, 2 cycles with 15 sec dwell | Bead Ruptor Elite | 19-620 |
Bull bone (Bos Taurus) | 5 g | DNA, RNA | 25 mm milling ball x 1 | 50 mL milling jar | 1. break bone (5g) into shards to fit milling jar with ball. 2. Place jar in liquid nitrogen (3 mins) 4. Load and process 25 Hz, 2 mins |
Bead Ruptor 96 | 27-006, 27-206 |
Cow/Bovine Articular cartilage | 5 mg | Protein | 2.8 mm ceramic | 2 mL tube | 1. Add 0.5 mL Tris. 2. 6 m/s for 30 secs, 2 cycles with 30 sec dwell |
Bead Ruptor Elite | 19-628 |
Coyote hair (Canis latrans) | 2-3 mm | Hormones | 2.8 mm ceramic | 2mL reinforced tube | 1. 6.8 m/s for 50 secs, 4 cycles | Bead Ruptor Elite | 19-628 |
Dog placenta (Canis familiaris) | 1 g | Small molecules | 2.8 mm ceramic | 7 mL tube | 1. Add 2 mL of water 2. 6 m/s for 30 sec |
Bead Ruptor Elite | 19-678 |
Horse hair (Equus ferus caballus) | 100 mg | Small molecules | 2.8 mm ceramic | 7 mL tube | 1. HIGH speed for 30 secs, 5 cycles with 30 sec dwells | Bead Ruptor 12 | 19-678 |
Horse hair (Equus ferus caballus) | 100 mg | Small molecules | 2.4 mm metal | 7 mL tube | 1. 6.8 m/s for 50 secs, 3 cycles with 3 min dwells | Bead Ruptor Elite | 19-670 |
Mouse lung (Mus musculus ) | 200 mg | Bacteria | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 1 mL Tris. 5.65 m/s for 30 sec, 3 cycles with 5 sec dwell |
Bead Ruptor Elite | 19-628 |
Mouse lung, kidney, liver, heart (Mus musculus ) | 25 mg | RNA | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 0.5 mL RLB buffer. 2. 2.9 m/s for 20 secs |
Bead Ruptor 12 | 19-628 |
Mouse quadricep muscle (Mus musculus ) | 50 mg | mRNA | 6.5 mm ceramic | 2 mL reinforced tube | 1. Add 0.5 mL PBS. 2. 4.5 m/s for 30 secs, 3 cycles with 20 secs dwell on ice |
Bead Ruptor Elite | 19-648, 19-682 |
Pig cartilage / porcine meniscus | 20 mg | protein | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 0.5 mL Tris 2. 6 m/s for 30 secs, 4 cycles with 30 secs dwell |
Bead Ruptor Elite | 19-628 |
Pig skin (Sus domesticus) | 200 mg | RNA | 2.8 mm ceramic | 7 mL tube | 1. Add 2 mL Tris. 2. 6 m/s for 30 secs, 3 cycles with 3 min dwells on ice |
Bead Ruptor Elite | 19-678 |
Pig skin (Sus scrofa domesticus) | 10 mm2 | RNA | 6.5 mm ceramic (one bead) | 7 mL tube | 1. Add 2.5 mL ice-cold TRIzol™. 2. 5.5 m/s for 30 secs, 2 cycles with 1 min dwell on ice |
Bead Ruptor Elite | 19-651-500, 19-682 |
Rat fat/adipose tissue (Rattus norvegicus) | 500 mg | Small molecule | 2.8 mm ceramic | 7 mL tube | 1. Add 3 mL water. 2. 6 m/s for 30 secs |
Bead Ruptor Elite | 19-678 |
Rat femur (bone) (Rattus norvegicus) | 200 mg | Small molecule | 2.4 mm metal | 7 mL | 1. HIGH speed for 1 min, 2 cycles with 1 min dwell on ice | Bead Ruptor 12 | 19-670 |
Rat brain (Rattus norvegicus) | 30 mg | Protein | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 750 µL Tris 2. Speed 4 for 45 secs |
Bead Ruptor 4 | 19-628 |
Rat brain (Rattus norvegicus) | 70 mg | Protein | 1.4 mm ceramic | 2 mL reinforced tube | 1. Add 500 µL TBS 2. 4.85 m/s for 20 secs |
Bead Ruptor Elite | 19-627 |
Rat brain (Rattus norvegicus) | 30 mg per well | Protein, DNA | 5 mm stainless steel (1 per well) | Deep 96-well plate | 1. Add 500 µL buffer 2. Seal plate with sealing mat 3. 20 Hz for 30 secs |
Bead Ruptor 96 | 27-7005, 27-520, 27-530 |
Rat colon (Rattus norvegicus) | 3.6 g | Small molecule | 2.8 mm ceramic | 30 mL tube | 1. Add 10 mL water 2. 5 m/s for 30 secs, 3 cycles with 1.5 min dwells |
Bead Ruptor Elite | 19-6358 |
Rat heart (Rattus norvegicus) | 50 mg | Protein | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 1 mL Tris 2. HIGH for 1 min |
Bead Ruptor 12 | 19-628 |
Rat heart (Rattus norvegicus) | 30 mg | Protein | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 750 µL Tris 2. Speed 4 for 45 secs, 2 cycles with 30 sec dwell |
Bead Ruptor 4 | 19-628 |
Rat heart (Rattus norvegicus) | 30 mg | Protein & DNA | 5 mm stainless steel (1 per well) | Deep 96-well plate | 1. Add 500 µL preferred buffer 2. 20 Hz for 30 sec, 3 cycles |
Bead Ruptor 96 | 27-7005, 27-520, 27-530 |
Rat small intestine (Rattus norvegicus) | 30 mg | DNA | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 200 µL lysis buffer 2. 5 m/s for 10 sec |
Bead Ruptor Elite | 19-628 |
Rat small intestine (Rattus norvegicus) | 30 mg | Protein, DNA | 5 mm stainless steel (1 per well) | Deep 96-well plate | 1. Add 500 µL preferred buffer 2. 20 Hz for 30 sec, 2 cycles |
Bead Ruptor 96 | 27-7005, 27-520, 27-530 |
Rat liver (Rattus norvegicus) | 30 mg | Protein | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 750 µL Tris 2. Speed 4 for 45 secs |
Bead Ruptor 4 | 19-628 |
Rat liver (Rattus norvegicus) | 70 mg | Protein | 1.4 mm ceramic | 2 mL reinforced tube | 1. Add 500 µL TBS 2. 5.3 m/s for 20 secs |
Bead Ruptor Elite | 19-627 |
Rat liver (Rattus norvegicus) | 5 g | Small molecule | 2.8 mm ceramic | 30 mL tube | 1. Add 15 mL water 2. 5 m/s for 45 sec, 2 cycles |
Bead Ruptor Elite | 19-6358 |
Rat liver (Rattus norvegicus) | 3 g | Small molecule | 2.8 mm ceramic | 15 mL tube | 1. Add 4 mL water 2. 5.8 m/s for 45 sec |
Bead Ruptor Elite | 19-6158 |
Rat lung (Rattus norvegicus) | 68 mg | Protein | 1.4 mm ceramic | 2 mL reinforced tube | 1. Add 500 µL TBS 2. 5.5 m/s for 20 secs |
Bead Ruptor Elite | 19-627 |
Rat muscle (Rattus norvegicus) | 55 mg | Protein, DNA | 2.4 mm metal | 2 mL reinforced tube | 1. Add 750 µL Tris 2. HIGH for 45 secs |
Bead Ruptor 12 | 19-620 |
Rat muscle (Rattus norvegicus) | 30 mg | Protein | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 750 µL Tris 2. Speed 4 for 45 secs |
Bead Ruptor 4 | 19-628 |
Rat muscle (Rattus norvegicus) | 50 mg | Lipid | 2.8 mm ceramic | 2 mL reinforced tube | 1. Add 1 mL water 2. 5.65 m/s for 1 min, 2 cycles |
Bead Ruptor Elite | 19-628 |
Rat spleen (Rattus norvegicus) | 60 mg | Protein | 1.4 mm ceramic | 2 mL reinforced tube | 1. Add 500 µL TBS 2. 4.85 m/s for 20 secs |
Bead Ruptor Elite | 19-627 |
Rat trachea (Rattus norvegicus) | 2 – 3 mm2 (3 – 10 mg) | RNA | 1.4 mm ceramic | 0.5 mL tubes | 1. Add 250 µL Tris 2. Speed 4 for 60 secs, 2 cycles |
Bead Ruptor 4 | 19-626 |
Birds
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Chicken liver (Gallus gallus domesticus) | 2 g | Small molecule | 2.8 mm ceramic | 15 mL tube | 1. Add 3 mL of water 2. 6.45 m/s for 30 secs, 3 cycles with 1 min dwells |
Bead Ruptor Elite | 19-6158 |
Chicken liver (Gallus gallus domesticus) | 5 g | Small molecule | 2.8 mm ceramic | 30 mL tube | 1. Add 10 mL of water 2. 5 m/s for 45 secs, 2 cycles |
Bead Ruptor Elite | 19-6358 |
Chicken liver (Gallus gallus domesticus) | 200 mg | Small molecule | 2.8 mm ceramic | 7 mL tube | 1. Add 1.8 mL 50:50 Methanol:H2O 2. 5.3 m/s for 30 secs |
Bead Ruptor Elite | 19-678 |
Chicken egg shells (Gallus gallus domesticus) | 6 g | Nutritional analysis | 10 mm milling ball x 4 | 50 mL milling jar | 1. 30 Hz, 2 mins | Bead Ruptor 96 | 27-203, 27-006 |
Goose feather (Anser anser domesticus) | One feather (~100 mg) | Steroids | 2.8 mm ceramic | 7 mL tube | 1. Cut up feather to fit tube. 2. 6.6 m/s for 30 secs, 5 cycles with 3 min dwells |
Bead Ruptor Elite | 19-678 |
Goose feather (Anser anser domesticus) | One feather (~100 mg) | Steroids | 2.8 mm ceramic | 7 mL tube | 1. Cut up feather to fit tube. 2. HIGH setting for 30 secs, 5 cycles with 3 min dwells |
Bead Ruptor 12 | 19-678 |
Fish
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Trout head bones (Oncorhynchus mykiss) | 2 g | Parasite | 2.8 mm ceramic | 50 mL tube | 1. Add 20 mL of 0.5% pepsin-HCL. 2. 4.7 m/s for 30 secs, 3 cycles with 2 min dwells |
Bead Ruptor Elite | 19-6508 |
Insects
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Beetle (Tenebrio molitor) | 30 mg | DNA | 2.8 mm ceramic | 2 mL reinforced tube | 1. 350 µL preferred lysis buffer 2. Speed 4 for 30 sec |
Bead Ruptor 4 | 19-628 |
Fruit fly (Drosophila melanogaster) (Whole) | One fly (30 - 50 mg) | Protein, DNA | 1.4 mm ceramic | 2 mL tube | 1. Add 1 mL Tris 2. 6 m/s for 30 secs |
Bead Ruptor Elite | 19-627 |
Honey bee (Apis mellifera) (Whole) | 4 bees | DNA | 2.8 mm ceramic | 7 mL tube | 1. Add 2 mL water 2. 4 m/s for 30 secs, 2 cycles with 15 sec dwell |
Bead Ruptor Elite | 19-678 |
Mosquito (Aedes aegypti) (Whole) | ~50 mg | DNA | 2.4 mm stainless steel | Deep 96-well plate | 1. Add 1 mL CTL buffer (Omega-Biotek). 2. 24 Hz, 5 mins. Note: Rotate plate halfway through cycle. |
Bead Ruptor 96 | 19-640, 27-520, 27-530 |
Wolf spider (Allocosa apora) (Whole) | One spider (240 mg) | Protein | 1.4 mm ceramic | 7 mL tube | 1. Add 2 mL Tris 2. 4 m/s for 30 secs |
Bead Ruptor Elite | 19-677 |
Nematodes
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Vinegar eel (Turbatrix aceti) | 3 mL culture | DNA | 0.5 mm glass | 2 mL reinforced tube | 1. Spin down and resuspend in 200 µL preferred lysis buffer 2. Transfer to 2 mL tube with bead media 3. Speed 5 for 20 - 30 sec |
Bead Ruptor 4 | 19-622 |
Fungi
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Yeast (Saccharomyces cerevisiae) | 3 mL culture | DNA | 0.1 mm ceramic | 2 mL reinforced tube | 1. Spin down and resuspend in 480 µL of LD buffer and lyticase solution 2. Incubate at 30 °C, add 200 µL lysis buffer + 10 µL antifoam 3. Transfer to 2 mL tube with bead mix 4. 5 m/s for 30 secs, 2 cycles with 45 sec dwell |
Bead Ruptor Elite | 19-632D |
Yeast (Saccharomyces cerevisiae) | 40 mL culture | RNA | 0.1 mm ceramic | 2 mL reinforced tube | 1. Spin down and resuspend in 480 µL of LD buffer and lyticase solution 2. Incubate at 30 °C, add 350 µL lysis buffer + 10 µL antifoam 3. Transfer to 2 mL tube with bead mix 4. 4.2 m/s for 30 secs, 2 cycles with 45 sec dwell |
Bead Ruptor Elite | 19-632D |
Bacteria
Matrix | Amount | Analyte | Bead media | Vessel | Protocol | Omni unit | Consumable or accessory Cat # |
---|---|---|---|---|---|---|---|
Escherichia coli | 15 mL overnight culture | Protein | 0.1 mm ceramic | 2 mL reinforced tube | 1. Spin down and resuspend in 1 mL Tris 2. Transfer to 2 mL tube with bead mix 3. Speed 5 for 1 min |
Bead Ruptor 4 | 19-632D |
Escherichia coli | 3 mL overnight culture | DNA | 0.1 mm ceramic | 2 mL reinforced tube | 1. Spin down and resuspend in 200 µL Tris-EDTA and lysozyme buffer 2. Incubate at 37 °C, add 100 µL lysis buffer + 10 µL antifoam 3. Transfer to 2 mL tube with bead mix 4. 4.35 m/s for 45 secs |
Bead Ruptor Elite | 19-632 |
Escherichia coli | 1.6 mL overnight culture | DNA | 0.1 mm ceramic | 2 mL reinforced tube | 1. Spin down and resuspend in 200 µL Tris-EDTA and lysozyme buffer 2. Incubate at 37 °C, add 100 µL lysis buffer + 10 µL antifoam 3. Transfer to 2 mL tube with bead mix 4. Speed 4 for 45 secs |
Bead Ruptor 4 | 19-632D |
Escherichia coli | 6 mL overnight culture | DNA, RNA | 0.1 mm ceramic | Deep 96-well plate | 1. Spin down and resuspend in 400 µL Tris-EDTA and lysozyme buffer 2. Incubate at 37 °C 3. Split samples, 200 µL for DNA extraction and 200 µL for RNA extraction into wells of 96-well plate with bead mix 4. Add 100 µL lysis buffer + 10 µL antifoam to each well 5. 30 Hz for 2 min |
Bead Ruptor 96 | 27-6006 |