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Application Note

ChIP-seq analysis workflow from tissues using bead mill homogenization and PIXUL multi- sample sonication

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Genomic and epigenomic information is important to scientific research, including human disease research. NGS (next-generation sequencing) is a popular technology for its ability to multiplex hundreds and thousands of samples for high-throughput workflows. To obtain meaningful data, however, researchers must depend on the initial quality and quantity of starting sample materials, which could be less than ideal in most cases. Labs, therefore, also depend on a reliable sample prep method upstream their preferred library prep assays. In this application note, we review a robust bead mill homogenization and multi-sample sonication method taken through ChIP-Seq analysis.

For research use only. Not for use in diagnostic procedures.

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ChIP-seq analysis workflow from tissues using bead mill homogenization and PIXUL multi- sample sonication