Live cell analysis of tumorsphere formation and clonogenic assays to quantify size and number of formed tumorspheres.
- Directly image tumor spheroids in various microwell formats
- Non-invasive brightfield imaging allows the user to image the same plate over multiple days
- Perform a two-color fluorescent viability assay
Introduction
The Celigo Image cytometer has been developed to fully automate imaging and analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.
Image and quantify tumorsphere formation and clonogenic survival assays
Celigo software displays a plate map, image view of the whole well, and numerical data for the counted spheres. The green fill view indicates counted spheres.
Formation and counting of tumorspheres from various cell lines
Whole-well image and individually counted MDA-MB-436 formed tumorspheres (outlined in green).
Whole-well image and individually counted SK-BR-3 formed tumorspheres (outlined in green).
Whole well image and individually counted MCF-7 formed tumorspheres (outlined in green).
Quantification of methylene blue-stained clonogenic colonies in 6-well plates
MDA-MB-468 colonies were stained with methylene blue 18 days following siRNA knockdown. The plate was imaged on Celigo at 3 um/px.
Whole-well image of the control sample
Whole-well image and counted colonies of siRNA treated sample
Colony number of MDA MB468 cells following siRNA knockdown
For research use only. Not for use in diagnostic procedures.