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A comparative study of two immunoassay platforms to determine lentivirus titer for CAR-T development
This application note demonstrates a comparative quantification of the p24 titer in a lentiviral GFP control sample using Alliance HIV-1 p24 Antigen ELISA and p24 AlphaLISA immunoassay platforms.
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Development of pharmacokinetic (PK) assays for detecting biosimilars targeting TNF alpha using AlphaLISA
In this Application Note, AlphaLISA™ assays are used for detecting biosimilars targeting TNF alpha. Learn more.
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Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits
This application note demonstrates the performance of AlphaLISA bovine cytokine kits by measuring the cytokine release from antigenstimulated PBMCs isolated from cow blood and shows how cytokine measurements are critical for understanding cell mediated immune responses during vaccine development.
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Simultaneous detection of drug efficacy and toxicity by combining HTRF, AlphaLISA, or AlphaLISA SureFire Ultra with ATPlite
This application note demonstrates how compound’s mechanism of action and potential cytotoxic effects can be deciphered thanks to the combination of AlphaLISA™, HTRF™ or AlphaLISA™ SureFire® Ultra™ immunoassays with ATPlite™ 1step cell viability assay.
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Simultaneous measurement of chemokine/cytokine release and antibody-dependent cellular cytotoxicity upon binding with rituximab
Simultaneous detection of ADCC activity and release of chemokines and cytokines from activated NK cells is possible via the combined use of DELFIA TRF and AlphaLISA technologies.
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A fast and simple chemiluminescent assay for monitoring of DNA-protein interactions
In this application note, we use HepG2 nuclear extracts to demonstrate the binding of Sp1 and HNF1 transcription factors to tagged oligonucleotides containing required cognate response elements. Read this note to see the results and access detailed data compared to EMSA.
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Utilization of AlphaLISA technology to accurately detect asthma biomarkers in human serum
In this application note, AlphaLISA technology was used to quantify biomarker levels in serum samples, specifically Human IL-21, IL-33, and TNFα.
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Fc-Receptor binding ADCC assays utilizing AlphaLISA technology: Characterization of hIgGs and FcγRIIIa
This application note demonstrates how AlphaLISA assay can be used to determine the binding activity of human IgG Fc fragment to human FcγRIIIa and to study how other antibodies bind to FcγRIIIa.
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Tracking inflammasome activity via measuring IL-1β levels with AlphaLISA and HTRF
This application note demonstrates the utility of AlphaLISA™ and HTRF™ for the detection of IL-1β in cell supernatant and serum samples which can be used as a biomarker for inflammasome activity
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No-Wash IP1 assays are a powerful readout to characterize compounds modulating the FGFR signaling pathway in cancer drug research
This application note demonstrates that detecting the intracellular accumulation of IP1, mediated by FGFR-dependent activation of PLCγ1, can be a powerful alternative for characterizing compounds that modulate FGFR signaling in various cancer cell lines expressing FGFR1, FGFR2, and FGFR3.
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Characterizing chemokine receptor inhibitors with AlphaLISA SureFire Ultra, Alpha SureFire Ultra Multiplex and LANCE Ultra cAMP assays
This application note demonstrates how the SureFire Ultra and LANCE Ultra cAMP assays can be used for measuring inhibitors to CCR7 and CXCR2 cell surface receptors using a cellular model system where these receptors are overexpressed in CHO cells.
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cAMP AlphaScreen assay: a method for the pharmacological characterization and screening of Gαi coupled receptors in whole cells
The note demonstrates the utility of the AlphaScreen-based cAMP Assay kit for the pharmacological characterization and potential high-throughput screening of Gαi-coupled receptors.
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