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Application Note

Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits

Leptospirosis is an infectious disease caused by Leptospira bacteria, which can spread across different species of mammals. Transmission occurs through contact or consumption of food from infected domestic animals. In cattle, Leptospira infection leads to reproductive failure, affecting productivity and profitability in the dairy and beef industries. Vaccinations are widely used to prevent infection in animals and reduce transmission to humans.

This application note:

  • demonstrates the performance of AlphaLISA bovine cytokine kits by measuring the cytokine release from antigen stimulated PBMCs isolated from cow blood
  • shows how cytokine measurements are critical for understanding cell mediated immune responses during vaccine development.

For research use only. Not for use in diagnostic procedures.

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Antigen-stimulated PBMC cytokine release measured by AlphaLISA bovine cytokine kits

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