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NEXTFLEX HT Agrigenomics Low-pass WGS Kit

The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO.

Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.

Feature Specification
Automation Compatible Yes

The NEXTFLEX™ HT Agrigenomics Low-pass WGS kit is designed for the highly efficient conversion of DNA, from both high- and low-quality samples from breeders, into Illumina® and Element® compatible libraries and can be paired with analysis solutions from CURIO.

Curio Genomics is a bioinformatics software company focused on accelerating agriculture genomics research.

Product Variants
Barcodes: 1-24
Unit Size
24 rxns
Part #:
NOVA-5141-70
List Price
USD 293.76
Barcodes: 1-384
Unit Size
384 rxns
Part #:
NOVA-5141-71
List Price
USD 4,700.16
Barcodes: 385-768
Unit Size
384 rxns
Part #:
NOVA-5141-72
List Price
USD 4,700.16
Barcodes: 769-1152
Unit Size
384 rxns
Part #:
NOVA-5141-73
List Price
USD 4,700.16
Barcodes: 1153-1536
Unit Size
384 rxns
Part #:
NOVA-5141-74
List Price
USD 4,700.16

For research use only. Not for use in diagnostic procedures.

Overview

The NEXTFLEX HT Agrigenomics Low-pass WGS Kit is a new, streamlined workflow compatible with Illumina and Element Biosciences platforms. With a wide input range, it is suitable for all kind of plant and animal samples and can be run either manually or in automated workstation. Performance of the libraries has been tested with samples homogenized and extracted with Revvity solutions.

  • Robust – fast and easy protocol
  • Wide input range – from 1 ng to 500 ng
  • PCR-free compatible workflow
  • High throughput – up to 1,536 UDI barcodes available

Additional product information

Low-pass whole genome sequencing (LP-WGS) followed by imputation is becoming the preferred method for genotyping among plant and animal breeders. LP-WGS can capture a much broader spectrum of genetic variation compared to SNP arrays and is not limited by a fixed content, speeding up the selection process and improving the reliability of predictions.  The high computational demands associated with processing and imputing LP-WGS data make it less accessible for many research programs and breeding initiatives.

CURIO™ Genomics is a bioinformatics software company focused on accelerating agriculture genomics research. Using proprietary ultra-parallelized cloud architecture, the CURIO platform enables processing of large and complex genomic data sets in minutes.

Reasons breeders are choosing CURIO

Speed - Better results in minutes, not hours or days

Versatility - Works with any species with a reference

Flexibility - Massively scalable cloud-based solution

Transparency - Fully transparent and validated best-in-class algorithms

Convenience - Build your own reference panel using a simple interface

Efficiency - Simple and easy integration for fully automated workflows

Imputation horizontal


Figure 1: Example of imputation analysis detail performed by CURIO on a domestic pig sample (Sus scrofa)

Flexible multiplexing options: Up to 1,536 UDI adapters

Barcodes are supplied with the NEXTFLEX HT Agrigenomics Low-Pass WGS Kit. We offer 1,536 color-balanced NEXTFLEX UDI adapters that improve multiplexing capabilities for both low-level and high-level multiplexing needs.

Automation compatibility

The NEXTFLEX HT Agrigenomics Low-Pass WGS Kit. is compatible with multiple liquid handlers, including the Fontus™ NGS, Sciclone® G3 NGSx, Sciclone® G3 NGSx iQ™, and Zephyr® G3 NGS workstations.

Workflow
Automation compatibility workflow

 

Online demonstration

You can now schedule a one-on-one 30-minute online demonstration with our experts to cover everything you need to know about the the NEXTFLEX HT Agrigenomics Low-Pass WGS Kit protocol and data analysis with Curio. Please choose a timeframe that suits you best and we will provide you a Microsoft Teams personal link to join when the time comes. We look forward to speaking with you.

Specifications

Application
DNA-seq library prep
Automation Compatible
Yes
Barcodes
1153-1536
Shipping Conditions
Dual Temperature
Unit Size
384 rxns

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