The HTRF HIV p24 Detection kit is designed for the detection and quantification of HIV p24 in buffered solution or cell culture medium.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Feature | Specification |
---|---|
Application | Protein Quantification |
Dynamic Range | 33 - 10,000 pg/mL |
Limit of Detection | 12 pg/mL |
Sample Volume | 16 µL |
The HTRF HIV p24 Detection kit is designed for the detection and quantification of HIV p24 in buffered solution or cell culture medium.
For research use only. Not for use in diagnostic procedures. All products to be used in accordance with applicable laws and regulations including without limitation, consumption and disposal requirements under European REACH regulations (EC 1907/2006).
Immunodeficiency Virus (HIV) p24 is the 231 amino acid phosphorylated protein of the capsid forming the conical core of the virus, which encapsulates the genomic RNA-nucleocapsid complex. p24 is a cleavage product of the p55 Gag polyprotein by viral proteases. HIV p24 and its 55 kDa precursor play crucial roles in the assembly, maturation, and disassembly of HIV. e therapy, HIV-derived lentiviral vectors are useful and commonly implemented to deliver transgenes to mammalian cells. Assays against the p24 antigen have become a standard method for measuring lentiviral transduction efficiency.
Application |
Protein Quantification
|
---|---|
Brand |
HTRF
|
Detection Modality |
HTRF
|
Dynamic Range |
33 - 10,000 pg/mL
|
Limit of Detection |
12 pg/mL
|
Product Group |
Kit
|
Sample Volume |
16 µL
|
Shipping Conditions |
Shipped in Dry Ice
|
Target Class |
Biologics
|
Technology |
TR-FRET
|
Therapeutic Area |
Infectious Diseases
|
Unit Size |
500 Assay Points
|
16 µL of recombinant protein in diluent #5 were dispensed into a low volume white microplate before the addition of 4 µL of the premixed HTRF anti HIVp24 detection reagents. The HTRF signal was recorded after 2h incubation at RT.
Sample size | 16 µL |
Final assay volume | 20 µL |
Time to results | 2h at RT |
Detection limit | 12 pg/mL |
Dynamic range | 33 to 10,000 pg/mL |
Sample compatibility | Cell supernatant |
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