Cell line development process
The process of developing a cell line to produce a specific protein or antibody involves multiple stages, all of which can be greatly aided by the Celigo™ image cytometer.
Transfection
- · Determine transfection efficiency
- · Monitor stability of transfection
Stabilized outgrowth
- Monitor growth in each well
- Identify confluent wells for passaging
- Increase growth with media optimization
Monoclonality
- Verify efficiency of limiting dilution, or single cell dispensing
- Identify single cell per well
Colony outgrowth
- Confirm single colony
Robotic integration for cell line development
Whole well images: Single cell to single colony
Well with a single cell that grew into a single colony
The Celigo provides an optional robotic API which can be controlled by various automation scheduling software applications. The Celigo is ready for integration with multiple automation partners and can be coupled with robotic arms, automated incubators and liquid handlers.
The Celigo can be used through the whole process of cell line development.
- Compatible with 96-, 384- and 1536-well plates.
- Identify wells with a single colony to avoid the time-consuming and manual identification of clones by eye.
- Measures colony size using brightfield and aids the process of selecting wells for clonal expansion.
- Automate cell line generation process with the Celigo robotic integration.
Clonal validation and expansion
Confirmation of clonality is a critical step in the generation of biopharmaceutical cell lines. With superior brightfield image quality, the Celigo image cytometer reliably visualizes individual cells in a well, even if the cell is adjacent to the well wall. The Celigo allows for verification that a clone originates from a single cell. Typically, limiting dilution is performed in multi-well plates which are then repeatedly imaged on the Celigo over a period of time. When colony growth is seen after a few days of culture, it is then possible to retrospectively confirm that it grew out of a single cell.
Clonal validation on Celigo provides several benefits:
- Fast brightfield scanning of multi-well plates (384-well plate can be scanned in <5 min)
- Image segmentation for detection of single cells in a well
- Heatmap representation of the number of cells/well
- Growth tracking report for monitoring cell growth over time
- Efficient database management of images and data
Clonal validation on the Celigo image cytometer
Clonal expansion on Celigo image cytometer
Single CHO-S cell to single colony
Zoomed brightfield images of a single CHO-S cell expanding into a colony.
Automatic detection of single CHO-S cells on Celigo image cytometer
Single cell segmentation and heat map
Representative brightfield single cell segmentation (left) and
resulting heat map of the number of cells per well (right).
For research use only. Not for use in diagnostic procedures.