TotalSeq™ and DepleteX® technologies for efficient analysis of the cellular transcriptome

Proteins play a critical role in cellular function, and understanding their expression across individual cells is essential. TotalSeq™ oligo-conjugated antibodies enable simultaneous profiling of protein markers alongside transcriptomes in single cells using techniques like CITE-Seq. By combining protein and RNA readouts, researchers gain high-resolution insights into heterogeneous cell populations, surpassing RNA clustering methods and improving cell type identification.

However, challenges persist in single-cell RNA sequencing (scRNA-seq), where up to 50% of reads can be abundant transcripts which can hinder the detection of low-abundance transcripts. Enter the DepleteX ^® Single Cell RNA Boost Kit, based on a novel CRISPR-Cas9-based depletion technology that selectively removes uninformative sequences prior to sequencing, enhancing cell type resolution while reducing reads per cell. In this study, we explore the compatibility of TotalSeq ^™ and DepleteX ^® in established 10x Genomics workflows, aiming for a more efficient analysis of the cellular transcriptome.

Download our application note, TotalSeq and DepleteX technologies for efficient analysis of the cellular transcriptome, to see the data, full methods and experimental details.

For research use only. Not for use in diagnostic procedures.