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Technical Note

Optimize your HTRF cell signaling assays for tissues

Monitoring the phosphorylation and expression of key proteins in cells and tissues is a cornerstone of in vivo efficacy studies in preclinical stages.

This Technical Note provides Revvity’s exclusive HTRF and Western blot compatible guidelines to optimize your cell signaling assays. It’s time to get the most from your experiments!

Key highlights:

  • Sample preparation steps
  • Focus on data processing and analysis
  • Detailed case study of ERK phosphorylation in a mouse liver

For research use only. Not for use in diagnostic procedures.

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Optimize your HTRF cell signaling assays for tissues