Skip to main content
Menu
US
mtb-infection1920
Literature - Publication Review

Imaging and gene editing approaches highlight important role of autophagy in controlling Mtb infection

Macrophages are the primary host cells for Mycobacterium tuberculosis (Mtb) during its intracellular survival in humans, and it has recently been suggested that autophagy plays a role in the response of host macrophages to Mtb infection. This present study details how researchers at The Francis Crick Institute used a combination of CRISPR-Cas9 gene editing and high-content imaging approaches to explore the role of autophagy in controlling Mtb infection. The authors believe such studies could enable the development of novel therapies to treat TB.

  • Use of CRISPR-Cas9 gene editing to knockout autophagy genes in human macrophages.
  • Use of high-content screening (using the Opera Phenix) to monitor replication of Mtb strains in these macrophages

For research use only. Not for use in diagnostic procedures.

To view the full content please answer a few questions

By submitting my personal data, I acknowledge that Revvity Inc. and its affiliates (“Company”) will process my personal data provided above consistent with the Company’s Privacy Policy available here.

CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Download Resource

Imaging and gene editing approaches highlight important role of autophagy in controlling Mtb infection