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Application Note

Development of AlphaLISA technology assays for the detection of neutralizing antibodies in human serum

Administration of biological therapeutics may result in the development of an immune response in patients. It is important that the presence of anti-drug antibodies (ADA) are evaluated and correlated with any potential pharmacological or toxicological observations.

We report here the development of two AlphaLISA functional assays, a cell-based and a biochemical assay, for the detection of neutralizing ADA in human serum samples.

For research use only. Not for use in diagnostic procedures.

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Development of AlphaLISA technology assays for the detection of neutralizing antibodies in human serum