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Technical Note
Development of an AlphaLISA assay to measure a DNA-protein interaction with a strong affinity
DNA-protein interactions are popular targets for the development of novel therapeutic drugs. There are a variety of assay formats to measure binding events; however, efficient, highly sensitivity assays that can be used to study a large range of binding affinities are important for fully validating therapeutics drugs.
In this technical note, we show how to easily develop a robust AlphaLISA™ assay that measures a tight binding interaction between a protein and a DNA oligonucleotide.
For research use only. Not for use in diagnostic procedures.
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Development of an AlphaLISA assay to measure a DNA-protein interaction with a strong affinity