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Application Note

Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays

This note compares two immunoassay technologies: ELISA (enzyme-linked immunosorbent assay) and AlphaLISA™ (amplified luminescent proximity-based homogeneous assay).

ELISA, despite being the gold standard, is laborious and time-consuming due to numerous wash steps. In contrast, AlphaLISA™, a bead-based homogeneous immunoassay, can deliver comparable or superior results with less time, cost, and sample volume.

The study compared these platforms in two complex sample matrices and examined key performance parameters. Read to learn more.

For research use only. Not for use in diagnostic procedures.

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Detection of human matrix metalloproteinase 9 in complex sample matrices, a comparison of AlphaLISA and colorimetric ELISA immunoassays

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