chemagic cfDNA extraction kit for HD917 cfDNA reference standard

Advancing cfDNA analysis: Research insights from the chemagic™ cfDNA Extraction Kit for Mimix HD917 cfDNA Reference Standard

The analysis of cell-free DNA (cfDNA) in human plasma is a groundbreaking approach in molecular screening, particularly for detecting genetic alterations in cancer research. This application note explores the compatibility of the Revvity´s Mimix HD917 cfDNA Reference Standard with the chemagic cfDNA 5k Kit (CMG-134), highlighting its significance in enhancing liquid biopsy assays.

Research Objectives

This study aims to evaluate:

• The extraction efficiency of cfDNA using the chemagic cfDNA Kit compared to traditional methods.
• The integrity of cfDNA fragment profiles and allelic frequencies post-extraction.

Methodology

The research involved extracting cfDNA from a 2 mL sample of the HD917 reference standard using two different extraction methods. The focus was on:

• Extraction Efficiency: Measuring the yield of cfDNA obtained from each method.
• Fragment Size Analysis: Assessing the length profiles of cfDNA to ensure compatibility with downstream applications.

Conclusion

This study underscores the importance of using high quality cfDNA extraction methods in oncology research. The compatibility of the Mimix HD917 cfDNA Reference Standard with the chemagic cfDNA 5K Kit not only enhances extraction efficiency but also preserves the integrity of cfDNA for accurate genetic analysis. These findings support the advancement of non-invasive diagnostic techniques in cancer detection and monitoring.

For detailed insights and data, download the full application note or contact us for further information.

For research use only. Not for use in diagnostic procedures.