Skip to main content
Menu
US
Application Note

Bead mill homogenization and chemagic 360 automated DNA extraction workflow for High-Definition PCR detection of tick-borne pathogen

tick-borne-pathogen-512x288

Ticks are considered one of the most dangerous arthropod vectors worldwide for the transmission of zoonotic infections, second only to mosquitoes. The increasing burden of tick-borne disease has prompted numerous public health efforts for research around mitigation and epidemiological surveillance, to better characterize pathogen burden in a given population. In this application note, whole ticks were mechanical disrupted via bead-beating on the Omni Bead Ruptor Elite bead mill homogenizer, spiked with known copy numbers of pathogen control DNA, and taken through an automated nucleic acid extraction on the chemagic 360 extractor. Resulting eluates were taken through the high-definition PCR (HDPCR) tick-borne pathogen (TBP) research panel to assess extraction efficiency.

For research use only. Not for use in diagnostic procedures.

To view the full content please answer a few questions

By submitting my personal data, I acknowledge that Revvity Inc. and its affiliates (“Company”) will process my personal data provided above consistent with the Company’s Privacy Policy available here.

CAPTCHA
This question is for testing whether or not you are a human visitor and to prevent automated spam submissions.
Download Resource

Bead mill homogenization and chemagic 360 automated DNA extraction workflow for High-Definition PCR detection of tick-borne pathogen