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Application Note

3D tumor spheroid analysis method for HTS drug discovery using Celigo imaging cytometer

Inhibition of cancer cell proliferation in drug discovery research has not translated well from traditional two dimensional (2D) in vitro assays to in vivo studies. Most anti-cancer drug compound studies are performed in a tissue culture treated, 2D-assay format for the purpose of studying proliferation, viability, and apoptosis. Increasingly, scientific evidence is showing that growing cancer cells in the form of three-dimensional (3D) spheroids is more predictive of in vivo study outcomes than 2D cell culture formats.

For research use only. Not for use in diagnostic procedures.

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3D tumor spheroid analysis method for HTS drug discovery using Celigo imaging cytometer

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