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Cell Counting and Image Cytometry

Celigo Fluorescent Assays - Apoptosis

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Topic
Celigo Fluorescent Assays - Apoptosis
Celigo Fluorescent Assays - Apoptosis
Celigo Fluorescent Assays - Wound Healing
Cell Cycle
Co-culture
Internalization and Phagocytosis
Surface Proteins and Antibodies
Transfection and Transduction
Viability and Cell Health

The Celigo™ image cytometer performs high-throughput, whole-well imaging and quantitative data analysis in brightfield and up to four fluorescent channels for a wide variety of cell-based assays.


Live Cell Analysis of Cell Health on the Celigo has Several Advantages: 

  1. Easy-to-use interface to identify cell health
  2. Rapid scanning and analysis (scan and analyze a 96-well plate using 3 fluorescent channels in <12 min) 
  3. Works in multi-well plate formats (1536-well to 6-well)
  4. Advanced flow cytometry-like gating
  5. Apoptosis Introduction

Quick assessment of cell health is performed using the Celigo image cytometer with its easy-to-use applications that allow the simple and rapid analysis of cell viability and apoptosis in many diverse cell types. The cell viability application uses Calcein AM, propidium iodide and Hoechst to identify and count live, dead and total cell numbers, respectively. Similarly, the Phosphatidylserine (PS) Externalization application measures apoptosis by quantifying annexin V, propidium iodide and Hoechst to report apoptotic, dead and total cell counts, respectively.
 

Phosphatidylserine (PS) Externalization Application on Celigo image cytometer
Image of Apoptotic Jurkat Cells & Concentration Response Curve

Apoptotic Jurkat Cells & Concentration Response Curve

Representative image and concentration response curve of apoptotic Jurkat cells treated with camptothecin. 

For research use only. Not for use in diagnostic procedures

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