LentiBOOST™ Technology
Improve lentiviral transduction efficiency with LentiBOOST™ Technology
LentiBOOST is a highly effective, non-cytotoxic transduction enhancer for research and clinical application of lentiviral vectors in a wide range of cell types.
It acts as a universal receptor-independent manufacturing aid which facilitates fusion of lentivirus with the cell membrane, increased vector copy number, and significantly improved lentivirus transduction efficiency at lower MOI.
Benefits of the LentiBOOST technology for drug development:
- Improved lentiviral transduction efficiency (Delville et al. 2018)
- Increased and titratable vector copy number per cell to support lower manufacturing costs
- Plug and play: Integrates into transduction protocols
- Available as pharma grade for research and GMP grade for clinical applications
Benefits for drug development:
- Up to 90% improved lentiviral transduction efficiency
- Increased and titratable vector copy number per cell
- Easy-to-use
- Pharma and GMP grade batches
For research use only. Not for use in diagnostic procedures.
Lower the cost of goods for cell therapies
LentiBOOST™ transduction enhancer allows you to decrease the vector quantity required so that you can reduce the cost of goods for cell therapies. Researchers from University of Manchester and UCL have shown an almost 5-fold reduction of vector amounts while achieving equivalent efficacy in a GMP stem cell manufacturing protocol for HSCs (Ellison et al. 2024).
Improved transduction and optimal copy number per cell
Using LentiBOOST, customers have demonstrated improved transduction without impacting cell health across a range of cell types, including primary T-cells or HSCs which are known to be challenging.
In this example, the number of GFP-positive human CD 34+ PBSC transduced with lentivirus and LentiBOOST at various concentrations reached up to 80% at day 12 post transduction.
Figure 1: The amount of GFP positive human CD34+ PBSC was determined at day 3, 6, 9, and 12 post transduction at various concentrations of transduction enhancer (LB; 1.5 mg/ml to 4.5 mg/ml). Source: Hauber et al., 2018
LentiBOOST technology allows for adjustable and controllable vector copy numbers per cell allowing for optimization in accordance with EMA/FDA safety guidelines. This helps to facilitate the expression of your therapeutic protein when using various concentrations.
Figure 2: Effect of varying concentrations of LentiBOOST (1.5 to 4.5 mg/ml) on transduction efficiency with LV-GFP (MOI=10) in human CD34+ PBSC. The star (*) signifies p ≤ 0.05. Source: Hauber et al., 2018
A customer’s head-to-head comparison of LentiBOOST with other transduction enhancers showed that LentiBOOST had the strongest effect on the transduction of the human T cells across the three concentrations they tested. At the highest concentration the transduction enhancing effect of LentiBOOST was the strongest, with efficiency about 5-times more than transduction without an enhancer.
Effective in a wide range of cell types
LentiBOOST can be applied to a wide range of clinically relevant cell types, including CD34+ hematopoietic stem cells (HSC), mesenchymal stem cells (MSC), neuronal stem cells, primary T cells, hard-to-transduce murine T cells, NK cells, and fibroblasts. The technology is ideal for clinical transduction protocols for ex vivo gene therapies and CAR-T cell therapies.
Below we show the unit-fold increase in transduced cells when using LentiBOOST based on customer data. The range depends on the experimental setup, vector design, and transduction protocols.
No cytotoxicity observed and differentiation potential maintained
Optimizing transduction efficiency must not be done at the expense of cell health or functionality. This has been assessed in various studies and HSC treated with LentiBOOST demonstrated the same viability as the control cells.
Figure 3: Determination of cell viability and toxicity in a MTT assay at day 3, 6, 9, and 12 post transduction. Source: Hauber et al., 2018
At least as important as health is the differentiation potential of the cells. Experiments have shown that LentiBOOST does not affect the ability of HSC to differentiate into various hematopoietic lineages (Hauber et al. 2018).
Successful track record
LentiBOOST™ has been integrated into more than 40 Phase I/II and III clinical trials, is used in 2 approved cell and gene therapy products, and has been used by over 600 academic and industry customers worldwide (as of May 2024).
Here is a selection of our LentiBOOST users:
- Mustang Bio (NCT01306019): Their MB-207 program aims to assess a lentiviral therapy for the treatment of patients with X-linked severe combined immunodeficiency. The clinical development is a Phase 1/2 study (as of end 2021). More here
- University of California San Francisco (NCT03538899): The goal of this study is to treat patients with Artemis-deficient Severe Combined Immunodeficiency. Patients receive their own transduced stem cells to restore the function of their immune system by introducing a normal copy of the DCLRE1C gene. This treatment is assessed in a Phase 1/2 study (as of end of 2021). More here
- National Institute of Allergy and Infectious Diseases: Their program aims to treat patients with X-linked severe combined immunodeficiency (SCID-X1). Patient CD34+ cells are transduced to express a healthy version of the gene ILR2G that is impaired in patients suffering from SCID-X1. More here
- Orchard Therapeutics: The biopharmaceutical company licensed the LentiBOOST technology to include in its gene therapy products to treat patients with rare diseases. More here
- Cellectis: The biopharmaceutical company licensed LentiBOOST technology to expand its portfolio of technologies for manufacturing its allogeneic CAR-T cells. More here
- Beam Therapeutics: The US company licensed LentiBOOST for use in their new generation of CAR-T product candidates using proprietary base editing technology. More here
LentiBOOST pharma grade for research use
LentiBOOST is available globally in pharma grade for research and process development purposes. Revvity supplies LentiBOOST pharma grade (non-GMP) material for research and process development including large-scale runs. LentiBOOST pharma grade is used in hundreds of laboratories worldwide.
LentiBOOST GMP grade for clinical use
LentiBOOST GMP grade material is available for clinical development and for cell therapies at the commercial stage under a clinical or commercial license.
LentiBOOST helps to increase the response rate and overall success of clinical trials. By integrating LentiBOOST into their processes, our licensees can make their manufacturing more robust and have access to GMP material for both clinical and commercial use.
We know how important a reliable and secure supply chain is, especially in the commercial phase. That’s why we use an automated fill and finish process under GMP conditions in accordance with regulatory requirements. Our quality assurance process ensures compliance with highest standards and batch-to-batch consistency. LentiBOOST GMP grade comes with a CoA and all necessary documentation for regulatory authorities such as the FDA and EMA.
We work closely with our licensees as a partner on the road to clinical approval of their cell therapy. To help advance your clinical success, we offer individualized licensing models aligned to help meet your needs.
Revvity offers two main types of licenses:
- Commercial licenses for companies that are developing and commercializing cell therapy programs.
- Academic licenses for investigator-led clinical trials: To support pioneers developing new cell therapies, Revvity offers an academic license for the LentiBOOST technology to academic partners to use in their clinical programs, providing that the non-profit research organization is responsible to the regulatory authorities for the clinical trial.
How much LentiBOOST do I need?
Material is supplied at a concentration of 100mg/ml. For most applications, LentiBOOST is applied at a dilution of 1:100 to 1:400.
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