GMP Workflows

Celigo is a plate-based benchtop brightfield and fluorescent imaging system designed for whole-well live-cell analysis and cell sample characterization. The Celigo system images and analyzes cells in various types of vessels including 6 – 1536 well plates, T25, T75 flasks, 10 cm dishes, and glass slides without disturbing their natural state. Individual cell level analysis is easily generated, providing cell level insights unlike ELISA or protein-based assays, and at a faster rate than flow cytometry. A broad range of complex cell-based assays have been optimized for the Celigo cytometer: <ul> <li>Apoptosis</li> <li>Cell cycle</li> <li>Fluorescent reporters</li> <li>Cytotoxicity</li> <li>Label-free proliferation</li> </ul> <strong>Rapid whole-well imaging and analysis</strong> <ul> <li>Sensitive whole-well imaging ensures accurate cell population analysis <ul> <li>Remove non-uniform seeding from the equation</li> <li>Accurately detect, image, and count every cell in every well</li> </ul> </li> <li>Specialized scanning mirrors enable fast, high-quality images</li> </ul> <strong>Advanced brightfield imaging and four fluorescent channels</strong> <ul> <li>Proprietary algorithm for simultaneous imaging and label-free cell analysis</li> <li>Perform real-time multiplex assays with four fluorescent channels</li> </ul> <strong>Convenient workflow designed for biologists</strong> <ul> <li> Multiple object-driven algorithms and a variety of assay-based applications <ul> <li> Accessible adaptation with easy-to-follow software </li> <li> Save customizable experiments as well as default settings </li> <li> Built-in gating parameters allow easy data analysis and visualization </li> <li> Real-time graphic feedback allows multiple parameters to be measured simultaneously for intuitive analysis </li> </ul> </li> </ul> <strong>Automation and data management</strong> <ul> <li>Accessible application programming interface (API) for easy automated workflow integration</li> <li>Automated microplate handling for kinetic end-point analysis or time-point analysis</li> </ul>

The Cellaca PLX system with Matrix analysis software and dedicated reagents and consumables provides a benchtop solution for accurate measurements of small sample volumes to easily perform rapid subpopulation analysis for downstream processing. <strong>Multiplexing Made Easy</strong> The Cellaca PLX image cytometry system is easy to use, performing simple yet sensitive cell counts, viability readouts, and multiplex analysis in seconds. <strong>Small Sample Volume</strong> The sample-efficient Cellaca PLX requires between 15 – 50 uL per single sample analysis. That’s 10-30 times less sample volume required per test, compared to a flow cytometer. <strong>Speed with Sensitivity</strong> The time to downstream processing is shortened by multiplexing with four channels with viability readouts at one minute per sample. <strong>Optimized Assays and Kits</strong> Our simple-to-use, mix, incubate, wash, and read reagent kits provide immunophenotyping plus viability multiplexing assays using disposable, low fluorescence consumables. <strong>User-Friendly Software</strong> Optimized protocols streamline surface marker staining, vitality, and apoptosis analysis with step-by-step methodologies and customizable result presentations. <strong>Low Auto-Fluorescent Consumables</strong> Allow for accurate surface marker detection. <strong>Hardware, Instruments, and System Capabilities</strong> The Cellaca PLX’s ability to consistently image, de-cluster, and report cell counts, viability, concentration, and phenotyping data makes it a versatile instrument during every stage of cell line development. The Cellaca PLX allows labs to shorten the time to downstream processing by multiplexing with up to four channels to obtain critical viability readouts at one minute per sample with no warm-up time. The cell population data can then be viewed as a histogram, scatter plot, dot plot, or contour plot for easy data viewing and analysis. Whether using it as a complementary method for additional data or as the first stop before starting downstream assays, image cytometry can save laboratories time and prevent excessive sample loss. With this output speed, researchers can quickly perform cell purity checks at the bench and then easily continue with downstream assays without waiting for a flow cytometer. By performing multiplexing assays at the bench, laboratories can reduce the reliance on a busy flow core facility, saving flow cytometry for more complicated or detailed assays. <strong>Expert Support for Assay Development</strong> On-site, remote, and hybrid training are available to assist with initial setup and continued assay support for improved workflows. <strong>Remote and Field Serviceability</strong> Calibration-free instrumentation with minimal routine maintenance. No clogging or liquid waste stream. <strong>Automation Ready</strong> Compatible with robotic integration and automated liquid handling systems. <strong>21 CFR Part 11 Ready</strong> <ul> <li>Audit trails, time stamping, user access control, and electronic signatures.</li> <li>File locking and change tracking.</li> <li>Creation of new customizable defined roles with multiple configurations available</li> <li>A close-looped database system to aid with data integrity and security.</li> <li>Stress-Free Intuitive Software</li> </ul> <strong>Built-in assays with optimized settings for over 400 individual cell types.</strong> Configurable data and calculation reports with graphs, images, charts, and tables for ease of information sharing. <strong>Low Auto-Fluorescent Consumables</strong> Cellaca PLX consumable slides provide a safe and self-contained environment for the sample which then does not require a decontamination procedure of the instrument at the end of each run. The slide holder, which is included with the instrument, can accommodate 4 slides or 8 samples per experimental run. <strong>Cellaca PLX Optimized Assay Reagents and Kits</strong> The Cellaca PLX system provides flow-like data with simple-to-use, mix, incubate, wash, and read reagent kits that provide immunophenotyping plus cell counts and viability multiplexing assays using disposable, low fluorescence consumables. Quickly perform cell counts, concentration, purity checks, and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay. <strong>Available assay kits include:</strong> <ul> <li>Immune cell Phenotyping</li> <li>Apoptosis detection</li> <li>High-throughput cell viability</li> <li>Fluorescent protein analysis</li> </ul> <strong>Cellaca PLX Applications</strong> With the Cellaca PLX Platform, you can: <ul> <li>Quickly perform cell purity checks and viability at the bench with Cellaca PLX immunophenotyping kits which combine CD3, CD4, and CD8 surface marker detection with cell viability in a single multiplex assay.</li> <li>Stain transfected cell lines with viability dyes to determine the percentage of transfected or transduced cells that are live/dead.</li> <li>Perform routine viability measurements as well as apoptosis functional assays to determine cell health.</li> </ul>