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Cell Counting and Image Cytometry

Measure Apoptosis

Section
Celigo Applications
Celigo Applications
Cell Counting Method Selection
Cell Counting and Image Cytometry FAQs
Cell-based Assays for Bioprocessing
Cell-based Assays for Gene Therapy Development
Cellometer Applications
Modern Virology Assays
Sub Section
3D Cell Models
3D Cell Models
Assays for Virology Research
Brightfield Applications
Celigo Cell Counting Applications
Celigo Fluorescent Assays
Celigo Image Cytometer Direct Cell Counting Assays for Immunotherapy
Cell Line Development
Migration and Invasion Assays
iPSC Reprogramming
Topic
Measure Apoptosis
Embryoid Bodies and Patient-Derived Organoids
3D Confrontation Assays
Growth Inhibition
Measure Apoptosis
Measure Migration onto extracellular matrix
Quantify Invasion into Matrigel
Tumor Spheroid Viability
Tumorsphere Formation & Clonogenic Survival
  1. Directly image tumor spheroids in various microwell formats
  2. Non-invasive brightfield imaging allows the user to image the same plate over multiple days
  3. Perform a two-color fluorescent viability assay

Introduction

The Celigo™ image cytometer has been developed to fully automate live cell analysis of tumorspheres. This automated morphometric analysis tool significantly reduces the time and effort needed to quantify key aspects of 3D spheres including size, growth, growth tracking over time and response to chemotherapeutics.

growth inhibition celigo image cytometer

Measure an increase in caspase 3/7 activity in HCT116 3D tumor spheroids

 

measure an increase in caspase

HCT116R2 and HCT116 spheroids were stained with Caspase 3/7 reagent and imaged. The above images present a merge of brightfield and Caspase 3/7 (green) images.

For research use only. Not for use in diagnostic procedures.

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