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Application Note Icon   Application Note
Measuring strong to weak binding interactions with AlphaLISA
In this application note, we demonstrate how AlphaLISA can be used to measure three very different types of biomolecular interactions with three very different binding affinities.
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cAMP assay provides flexibility and stable pharmacology
AlphaScreen™ technology provides a quantitative detection of molecules in a homogeneous, no-wash format and can be applied to GPCR research.
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Functional GPCR Studies Using AlphaScreen cAMP Detection Kit
Read this application note to access detailed data and results on GPCR studies using AlphaScreen cAMP detection kit
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Avoiding biotin interference in AlphaLISA assays
This application note demonstrates the value of using AlphaLISA biotin-free kits to reduce the effects of biotin interference in sample and standard preparations.
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Measure more analytes in each well.
Now you can quantify multiple analytes in a single well for faster and more accurate results, even with low sample volumes
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Quantify low abundant mIL-17A homodimer in mouse serum and plasma
This application note demonstrates the quantification of low levels of mIL-17A homodimer in mouse serum and plasma samples using the AlphaLISA mIL‑17A homodimer detection kit.
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Comparison of EMT Biomarker Expression in 2D Monolayer and 3D Spheroid Cultures in a Prostate Cancer
This application note shows that AlphaLISA can be used to compare differences in protein expression levels and cellular tolerance for compound treatment between a human prostate cancer cell line grown in monolayers and those same cells grown in 3D spheroids.
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Measuring Performance of an Automated and Miniaturized LANCE Ultra cAMP Assay for the Gi-coupled 5-HT1A Receptor- a Comparative Study
In this note we describe the miniaturization of a LANCE Ultra cAMP assay from 384-to 1,536-well plate format for the identification and characterization of agonists and antagonists of a Gi-coupled GPCR.
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LANCE Ultra TR-FRET-based detection and modulation of phosphorylated STAT3 levels in human cells.
STAT3 is a particularly important therapeutic target since it is constitutively active in a range of cancer cells lines and human tumors. Therefore, fast and easy methods for screening of inhibitors of STAT3 activation are highly desirable.
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2024 Fresh Lot Calendar
2024 Fresh Lot Calendar
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Proliferation and cell death analyses of 3D cultures using Revvity CellCarrier Spheroid ULA microplates and ATPlite 3D products.
In the process of developing new therapeutic molecules, toxicity – whether it is to be avoided or whether it is desired – is an important feature to consider as early as possible in the development flow.
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Biochemical binding ADCC assays utilizing AlphaLISA toolbox reagents for the characterization of hIgGs and FcγR1A
In this application note we demonstrate the ease with which AlphaLISA toolbox reagents can be used to develop any FcγR binding assay across the various stages of biologics research and development, including therapeutic screening and GMP Lot Release.
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