Opera Phenix Plus High-Content Screening System

Revvity Sites Globally

Select your location.

*e-commerce not available for this region.

Australia

Austria

Belgium

Brazil *

Canada

China *

Denmark

Finland

France

Germany

Hong Kong *

India *

Ireland

Italy

Japan *

Luxembourg

Mexico *

Netherlands

Norway

Philippines *

Republic of Korea *

Singapore *

Spain

Sweden

Switzerland

Thailand *

United Kingdom

United States

Opera Phenix Plus High-Content Screening System

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Fluor 641 Mitochondrial stain. Imaged on Opera Phenix Plus system, 40x water immersion objective.

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Fluor 555-WGA & PhenoVue Fluor 568-Phalloidin. Imaged on Opera Phenix Plus system, 40x water immersion objective.

U2OS Cells Stained with PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

U2OS Cells Stained with PhenoVue 512 Nucleic Acid Stain. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

Composite image of HeLa cells (untreated) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS Cells Stained with PhenoVue Fluor 488 Concavalin A. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

MRC5 live cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain (tubulin, red) & Phenovue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Opera Phenix Plus.

U2OS cells stained with PhenoVue 561 Mitochondrial Stain (Orange), PhenoVue Fluor 647 Live Cell Tubulin Stain (Red) and PhenoVue Hoechst 33342 (Blue).

U2OS Cells Stained with PhenoVue Fluor 641 Mitochondrial Stain. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

U2OS live cells stained with PhenoVue 551 Mitochondrial Stain (mitochondria, orange) & PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Opera Phenix Plus.

Opera Phenix Plus High-Content Screening system with liquid handling module and fast imaging frame rate for fast-response assays.

Composite images of HeLa cells (treated with cytochalasin) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

COS7 cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain & PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus.

CaFlux Assay iPSC-dervied neurons (nexel) stained with Cal-520 on Opera Phenix Plus (20xW).

Composite images of HeLa cells (treated with CA74) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

C2C12 cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain & PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus.

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Fluor 488 Concavalin A. Imaged on Opera Phenix Plus system, 40x water immersion objective.

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue Hoechst 33342 Nuclear Stain. Imaged on Opera Phenix Plus system, 40x water immersion objective.

Zebrafish 72hpf – Neutrophils. Lateral positioning on agarose. Imaged by PreciScan on Phenix Plus, 20xAir, Widefield : Brighfield / Ds Red (MIP). Image provided by Institut Pasteur Paris (PBI).

Composite images of HeLa cells (treated with tetrandine) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS live cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain (tubulin, red) & PhenoVue Hoechst 33342 Nuclear Stain (nucleus, blue). Imaged on Opera Phenix Plus.

Composite images of HeLa cells (treated with berberine) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

Picture of HeLa cells treated with etoposide

Opera Phenix Plus High-Content Screening system for fast response assays, phenotypic screening, and assays using complex cell models.

Opera Phenix Plus High-Content Screening system for high-throughput high-content assays.

U2OS Cells Stained with PhenoVue Fluor 555 WGA & PhenoVue Fluor 568 - Phalloidin. Imaged on Opera Phenix Plus, confocal, 40x water immersion objective.

Composite images of HeLa cells (treated with fembendazole) stained with the PhenoVue Cell Painting Kit. Imaged on Opera Phenix Plus, 40x water immersion objective.

U2OS cells stained with PhenoVue Fluor 568 - Phalloidin, PhenoVue Fluor 555 - WGA, PhenoVue Fluor 488 - Concanavalin A, PhenoVue Fluor 641 Mitochondrial Stain, and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus, 63X Water Confocal.

U2OS cells stained with PhenoVue Fluor 647 Live Cell Tubulin Stain & PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus, 63X water objective confocal.

HeLa cells stained with PhenoVue 551 Mitochondrial Stain

U2OS live cells stained with PhenoVue 551 Mitochondrial Stain (mitochondria, orange). Imaged on Opera Phenix Plus.

CRISPR Knockout Aurora Kinase A. U2OS CAG-Cas9 stable cell line. Stained with PhenoVue 512 Nucleic Acid Stain. Imaged on Opera Phenix Plus system, 40x water immersion objective.

U2OS cells 5-Plex staining: PhenoVue Hoechst 33342 (blue), PhenoVue Fluor 400LS - Phalloidin (fuchsia), PhenoVue Fluor 488 - Concanavalin A (green), PhenoVue Fluor 555 - WGA (orange), PhenoVue 641 Mitochondrial (red)

U2OS stained with PhenoVue Fluor 400LS – Phalloidin displayed in orange or fuchsia pseudo colors and PhenoVue Hoechst 33342. Imaged on Opera Phenix Plus.

Opera Phenix Plus High-Content Screening System

The Opera Phenix™ Plus high-content imaging system is a premier confocal solution for today’s most demanding high content applications. Drawing on over two decades of experience, the Opera Phenix Plus is designed for high-throughput high-content imaging assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells and microtissues, and fast-response assays, such as Ca²⁺ flux.

For research use only. Not for use in diagnostic procedures.

View product information

Product Variant

Unit Size: 1 Unit

Part #:

HH14001000

Request a Quote Request More Information

Product information

Overview

Unlock Deeper Insights with Opera Phenix Plus!

Discover More: High content imaging provides a greater depth of information than other approaches, allowing you to explore cellular processes in detail

Quantify in Detail: Analyze phenotypic changes at scale, from single cells to entire populations. High-content imaging empowers detailed quantification.

Enhance 3D Imaging: Water immersion objectives elevate 3D image quality, revealing intricate details within complex biological structures.

Boost Throughput: Add more cameras to increase speed and efficiency. High-content imaging accelerates your research pipeline.

Make meaningful discoveries faster with our advanced high-content imaging solutions.

Additional product information

Applications

Micro Physiological Systems (MPS)

Deepen understanding of 3D cell models with clearer images that reveal critical features and processes.

Functional genomic screening

Create genetic evidence with ease by phenotyping cellular response to thousands of gene manipulations in a single experiment.

Cell painting

Simplify multiparametric screening with out of the box reagents and analysis to reduce complexity (and create more time for you to focus on the science).

Drug sensitivity testing

Determine rational treatment selections regimens by rapidly characterizing ex vivo cellular function in response to drug treatment.

Fast response assays

Capture rapid cellular events and dynamic processes with fast frame rates and on-board liquid dispensing.

Features at a glance

multiple cameras

Multiple cameras

Increase imaging speed by multiple cameras and simultaneous acquisition especially for extensive stacks for 3D models.

microlens pinhole disk

Microlens-enhanced pinhole disk

Microlenses increase the excitation efficiency allowing for a bigger pinhole to pinhole distance thereby reducing out of focus light in confocal imaging.

automated water immersion objectives

Automated water-immersion objectives

Improve image quality and get better data by enhancing the signal and improving the z resolution while capturing more light.

proprietary synchrony optics

Proprietary Synchrony™ Optics

Combine a microlens-enhanced pinhole disk with dual-view confocal optics. This minimizes spectral crosstalk during simultaneous acquisition by separating fluorescence excitation and emission. Provide greater speed and higher sensitivity.

machine learning

Machine learning

Easily create algorithms without being an image analysis expert using our PhenoLOGIC proprietary machine-learning technology.

intelligent image acquisition

Intelligent image acquisition

Image only the objects you are interested in, centered at high magnification, thereby reducing imaging time and data size.

powerfully simple analysis

Powerfully simple analysis

Harmony™ high-content imaging and analysis software can make you more productive faster with ready made templates and simple steps to a custom analysis

3d analysis

3D analysis

Explore your cell models by visualizing them in a 3D- and an XYZ-viewer and quantify volumetric and other 3D related phenotypic readouts.

Opera Phenix Plus family – typical configurations

Opera Phenix Plus Single

The same sensitive and resolution as the rest of the Phenix family, with the ability to upgrade later with additional cameras.

Opera Phenix Plus Simultaneous

Higher speed, dual-camera system for multi-color, simultaneous confocal image acquisition and fast multiplexing.

Opera Phenix Plus FRET

With its five lasers and four-camera setup, it supports CFP/YFP FRET applications to map protein-protein interactions.

Opera Phenix Plus Screener

The ultimate in throughput and performance, it delivers four cameras and four higher powered lasers-supporting screening of large libraries.

		Single	Simultaneous	FRET	Screener
System Options	Number of cameras	1	2	4	4
	Camera upgrade path	2 or 4 cameras	4 cameras	-	-
	Automated water immersion lenses	✔	✔	✔	✔
	Transmitted light	✔	✔	✔	✔
	Environmental control	✔	optional	✔	optional
	On-board liquid handling	optional	optional	optional	optional
	Robotics/Automation Compatible	✔	✔	✔	✔
	Emission filters	8	up to 16	up to 14	up to 14
Acquisition speed	2D/3D	+/+	++/++	+++/++++	+++/++++
Lasers	375/425 nm	-	-	✔	-
	405 nm	✔	✔	-	✔
	488 nm	✔	✔	✔	✔
	561 nm	✔	✔	✔	✔
	640 nm	✔	✔	✔	✔
Imaging modes	Synchrony optics for minimized simultaneous imaging cross talk	-	✔	✔	✔
	Multi-color fluorescence imaging	✔	✔	✔	✔
	Multi-color simultaneous confocal imaging	-	✔	✔	✔
	3D imaging	✔	✔	✔	✔
	Brightfield and digital phase contrast	✔	✔	✔	✔
	Fluorescence widefield imaging	✔	✔	✔	✔
	Ratiometric FRET*	+	+	++	+
	Fast frame rate imaging	✔	✔	✔	✔

*CFP/YFP or spectrally similar pairs

Image aquisition and analysis simplified

Intuitive workflow: Harmony™ software offers an intuitive user interface that guides you from image acquisition to analysis and evaluation.
Templates for quick set-up: Templates allow you to set up acquisition channels and parameters efficiently.
Ready-made solutions: Choose from pre-built solutions for common image analysis tasks, simplifying your workflow.
Customizable building blocks: Create, configure, and customize your own high-content analysis applications using image analysis building blocks.
Advanced features: Harmony™ includes advanced analysis capabilities, such as texture and STAR morphology analysis, providing detailed descriptions of cellular morphology and robust differentiation of phenotypes.
Data management: The software automatically stores analysis results and metadata, including assay layout, instrument settings, and user-defined keywords and annotations.

Progress faster with verified solutions

Microplates for high-content imaging:
- Utilize PhenoPlate™ 384-well microplates designed for optimal performance in high-content imaging applications.
- Employ CellCarrier Spheroid ULA plates for imaging 3D models.
Automation and workstations:
- Improve throughput, productivity, and reduce variability and reagent costs by automating your Opera Phenix Plus system.
- Benefit from automated workflows using the explorer™ G3 workstations for cell painting, 3D cell culture, or phenotypic screening.
Efficient data management:
- Export results automatically into the Signals Image Artist™ platform that uses high performance computing and an industry standard object store to provide a scalable, multi-user solution for image analysis and management.
- Access, re-analyze, store, and share all your cell image data from Opera Phenix Plus and other high-content screening (HCS) systems.
Advanced analysis with signals vitro vivo:
- Perform screening data analysis and validation.
- Conduct QC analyses, calculate reliable normalization, multivariate hit stratification, dose-response curves, and drug response profiling.