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DOPlify Whole Genome Amplification V2 Kit
DOPlify Whole Genome Amplification V2 Kit
Revvity kit box for reagents and consumables
The DOPlify® WGA V2 kit performs fast whole genome amplification on single cells or limited template DNA samples, allowing for the cell chromosome copy number status to be determined.
For research use only. Not for use in diagnostic procedures.
| Feature | Specification |
|---|---|
| Product Group | Whole Genome Amplification |
The DOPlify® WGA V2 kit performs fast whole genome amplification on single cells or limited template DNA samples, allowing for the cell chromosome copy number status to be determined.
For research use only. Not for use in diagnostic procedures.
Product Variant
Unit Size: 50 rxn
Part #:
4321-0020
List Price
USD 1,059.00
DOPlify Whole Genome Amplification V2 Kit
Revvity kit box for reagents and consumables
DOPlify Whole Genome Amplification V2 Kit
Product information
Overview
Single cell whole genome amplification
The DOPlify® WGA V2 kit offers the advantage of full mitochondrial DNA coverage and the ability to add custom primers to specially amplify regions of interest. Products of the DOPlify® WGA V2 kit can be used for a wide range of downstream processes, workflows, and NGS applications. The technology captures exclusive know-how for the lysis and whole genome amplification, with reagents provided in an easy-to-use kit format and less than 3 hour protocol time.
- NGS compatible advanced DOP-PCR
- Fast whole genome amplification with protocol time < 3 hours
- Reduced contamination risk with only 2 tube openings required and 3 reagents to add
- Can be fully automated with the JANUS® G3 liquid handler
- No amplification of No Template Control
- 2.5 μg of DNA from one cell
Additional product information
Why Use DOPlify Technology?
The DOPlify WGA V2 kit is a whole genome amplification kit designed to PCR amplify the genome from single cells and limited DNA templates.
The DOPlify kit is unique in that it uses advanced DOP-PCR, which;
- uses the high processivity and fidelity of new generation polymerases that are NGS application grade
- uses ready-mixes to minimize the pipetting steps and requiring only two sample tube openings to reduce the risk of contamination
- provides an amplification system that is flexible enough to concurrently whole genome amplify a sample while specifically amplifying genes of interest, reducing the risk of allele drop-out.
DOPlify workflow
| Protocol Step | Explanation |
Time |
|---|---|---|
| Cell Lysis | A gentle but effective enzyme-based lysis procedure ensures robust cell lysis and a readily accessible DNA template for whole genome amplification |
15 mins |
| Whole Genome Amplification | Whole genome amplification is performed using advanced DOP-PCR, which has been optimized for limited DNA input. DOP-PCR uses degenerate primers to initiate DNA amplification, binding across a broad range of different sequences scattered genome wide |
2.5 hrs |
| Quality Control | Following amplification, the analysis of products by agarose gel electrophoresis or microfluidic electrophoresis is recommended to ensure that cell amplification has been successful |
30 mins |
Can DOPlify WGA V2 be automated?
The whole genome amplification of single or small numbers of cells and downstream processes such as library preparation for NGS can be fully automated with the JANUS G3 liquid handler.
Whole genome amplification (WGA) of single cells in tubes has traditionally been performed by hand, which can lead to multiple potential issues including:
- Errant volume pipetting
- Technologist fatigue
- Wasted time
- Sample contamination
Using the JANUS G3 liquid handler with preprogrammed, easy-to-use automated WGA protocols your lab can:
- Reduce human errors and contamination risk
- Gain time for your staff to perform more important tasks
- Increase your lab’s throughput with walkaway automation
- Ensure sample process traceability with barcode scanning and sample tracking
Revvity’s JANUS G3 liquid handler can be used to automated whole genome amplification using the DOPlify kit or other WGA technologies with a throughput of 48 - 96* samples per run.
* Higher throughput options are available
Specifications
| Product Group |
Whole Genome Amplification
|
|---|---|
| Shipping Conditions |
Shipped in Dry Ice
|
| Unit Size |
50 rxn
|
References
- Deleye, L., Tilleman, L., Plaetsen, A. V., Cornelis, S., Deforce, D., & Nieuwerburgh, F. V. (2017). Performance of four modern whole genome amplification methods for copy number variant detection in single cells. Scientific Reports, 7(1). doi:10.1038/s41598-017-03711-y.
- Deleye, L., Plaetsen, A. V., Weymaere, J., Deforce, D., & Nieuwerburgh, F. V. (2018). Short Tandem Repeat analysis after Whole Genome Amplification of single B-lymphoblastoid cells. Scientific Reports,8(1). doi:10.1038/s41598-018-19509-5.
- Mai, A. D., Harton, G. L., Quang, V. N., Van, H. N., Thi, N. H., Thuy, N. P., . . . Quoc, Q. T. (2020). Development and clinical application of a preimplantation genetic testing for monogenic disease (PGT-M) for beta thalassemia in Vietnam. Journal of Assisted Reproduction and Genetics. doi:10.1007/s10815-020-02006-y.
- Plaetsen, A. V., Deleye, L., Cornelis, S., Tilleman, L., Nieuwerburgh, F. V., & Deforce, D. (2017). STR profiling and Copy Number Variation analysis on single, preserved cells using current Whole Genome Amplification methods. Scientific Reports, 7(1). doi:10.1038/s41598-017-17525-5.
Resources
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Application Note
DOPlify v2 with PicoPLEX App Note
In this appplication note the DOPlify® v2 WGA kit and Takara® PicoPLEX® Single Cell WGA kit were compared and performed comparably...
Application Note
DOPlify WGA kit App Note
The DOPlify® kit from Revvity is a whole genome amplification kit designed and optimized specifically to amplify the genome from...
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