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Technical Note
AlphaLISA DOT1L histone H3 lysine-N-methyltransferase assay
Unlocking the secrets of epigenetics, our technical not dives into optimizing a DOT1L enzymatic assay.
This technical document outlines the optimization of a DOT1L enzymatic assay using oligonucleosomes as the substrate. Discover how the assay easily detects the dimethylated product of histone H3 lysine 79 by leveraging a biotinylated anti-H3K79me2 antibody, which binds to the epigenetic mark of interest.
For research use only. Not for use in diagnostic procedures.
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AlphaLISA DOT1L histone H3 lysine-N-methyltransferase assay