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Technical Note
AlphaLISA Di-Methyl-Histone H3 lysine 4 (H3K4me2) cellular assay
Decoding Histone Modifications: A Targeted Assay for H3K4me2.
In this study, you will find details about an optimized assay designed to quantify changes in H3K4me2 levels following treatment with sodium butyrate and Trichostatin A (TSA), both of which are non-selective histone deacetylase (HDAC) inhibitors. Using a standardized histone extraction procedure, we detect the target mark by employing a biotinylated anti-Histone H3 (C-terminus) antibody and AlphaLISA Acceptor beads conjugated to an antibody specific to the mark.
For research use only. Not for use in diagnostic procedures.
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AlphaLISA Di-Methyl-Histone H3 lysine 4 (H3K4me2) cellular assay