Advancing autophagy detection: HTRF LC3B-II assay for specific and reliable LC3B-II detection

Unlock precision and speed in autophagy quantification: Discover the HTRF LC3B-II advantage in this application note.

This application note validates an innovative HTRF LC3B-II immunoassay, comparing it to the standard LC3 Western Blot method. Using various cellular models, the assay showed high specificity and sensitivity for detecting LC3B-II. It correlated strongly with LC3-II levels from Western blotting and detected subtle changes in autophagic flux due to pharmacological treatments. The HTRF LC3B-II assay is a reliable tool for quantifying autophagy, offering advantages in speed, throughput, and precision over traditional methods.

This application note demonstrates:

• Performances comparison between Western Blot and immunoassay
• High specificity and sensitivity of HTRF LC3B-II immunoassay
• Subtle detection of changes in autophagic flux
• Numerous advantages in speed, throughput, and precision

For research use only. Not for use in diagnostic procedures.